Williams R C, Malone C C, Roemer J H, Silvestris F, Solomon A
Department of Medicine, University of Florida Health Sciences Center, Gainesville 32610, USA.
Clin Immunol Immunopathol. 1995 Dec;77(3):366-73. doi: 10.1006/clin.1995.1164.
We studied reactivity of affinity-purified human IgG anti-F(ab')2 antibodies for antigenic determinants on kappa light chains. Variable (VL) and constant (CL) regions of a human kappa I light chain (EU) were studied for enzyme-linked immunosorbent assay reactivity with monoclonal anti-k antibodies and human IgG antiF(ab')2 using overlapping heptamers of primary sequence synthesized as peptides on polypropylene pins. Polyclonal IgG anti-F(ab')2 antibodies isolated by affinity chromatography from the serum of patients with systemic lupus erythematosus (SLE) were found to react primarily with CDR1- and CDR3-related peptides. The most reactive residues included IL29, TRY32, LEU33, ALA34, GLU90, TYR91, and ASN 92. No striking difference in overall V-region anti-F(ab')2 reactivity profiles was noted when 10 IgG anti-F(ab')2 preparations from normal subjects and SLE patients were compared. In contrast, however, when tested against overlapping C kappa heptamer sequences, the reactivity in SLE-associated anti-F(ab')2 antibodies was markedly decreased. We report that this difference may reflect a defect in anti-idiotypic control mechanisms in SLE.
我们研究了亲和纯化的人IgG抗F(ab')2抗体对κ轻链上抗原决定簇的反应性。利用在聚丙烯针上合成的作为肽的一级序列的重叠七聚体，研究了人κI轻链（EU）的可变（VL）区和恒定（CL）区与单克隆抗κ抗体和人IgG抗F(ab')2的酶联免疫吸附测定反应性。通过亲和层析从系统性红斑狼疮（SLE）患者血清中分离出的多克隆IgG抗F(ab')2抗体，发现主要与CDR1和CDR3相关肽发生反应。反应性最强的残基包括IL29、TRY32、LEU33、ALA34、GLU90、TYR91和ASN 92。比较来自正常受试者和SLE患者的10种IgG抗F(ab')2制剂时，未发现总体V区抗F(ab')2反应性谱有明显差异。然而，相比之下，当针对重叠的Cκ七聚体序列进行测试时，SLE相关抗F(ab')2抗体的反应性明显降低。我们报告，这种差异可能反映了SLE中抗独特型控制机制的缺陷。
