Tadey T, Purdy W C
Department of Chemistry, McGill University, Montreal, Quebec, Canada.
Electrophoresis. 1995 Apr;16(4):574-9. doi: 10.1002/elps.1150160193.
The addition of sodium dodecyl sulfate (SDS) micelles in the running buffer can be used to resolve mono- and diphosphorylated isomers of the insulin receptor peptide by capillary electrophoresis. The effect of SDS on peptide resolution is very dependent on pH. Complete resolution of three monophosphorylated isomers is achieved in uncoated capillaries filled with phosphate buffer containing 25 mM SDS and buffered at pH 6.1. Resolution of the diphosphorylated isomers can be significantly improved by using polyacrylamide coated capillaries. In coated capillaries electroosmotic flow is negligible and the migration order of the isomers is reversed. This allows for a longer period of interaction between the diphosphorylated isomers and the micelle and therefore selectivity is improved. Efficiency of all peptide isomers was also improved in coated capillaries due to reduced adsorption to the capillary wall.
在运行缓冲液中添加十二烷基硫酸钠（SDS）胶束可用于通过毛细管电泳分离胰岛素受体肽的单磷酸化和双磷酸化异构体。SDS对肽分离的影响非常依赖于pH值。在填充有含25 mM SDS且pH值为6.1缓冲的磷酸盐缓冲液的未涂层毛细管中，三种单磷酸化异构体可实现完全分离。使用聚丙烯酰胺涂层毛细管可显著提高双磷酸化异构体的分离度。在涂层毛细管中，电渗流可忽略不计，异构体的迁移顺序相反。这使得双磷酸化异构体与胶束之间的相互作用时间更长，因此选择性得到提高。由于减少了对毛细管壁的吸附，所有肽异构体在涂层毛细管中的效率也得到了提高。
