Skandalis A, Curry J, O'Neill J P, Nicklas J A, Albertini R J, Glickman B W
Center for Environmental Health, University of Victoria, British Columbia, Canada.
Environ Mol Mutagen. 1995;26(3):213-7. doi: 10.1002/em.2850260305.
The mutagenic impact of various environmental and therapeutic agents can now be directly assayed in humans by the T-lymphocyte cloning assay. We have previously reported that following radioimmunoglobulin therapy, cancer patients exhibited increased mutant frequency of the hprt locus and an increased yield of large intergenic deletions compared to unexposed controls. Here we report the results of the analysis of 26 independent hprt mutations in nine cancer patients who underwent radioimmunoglobulin therapy. The majority of mutations (52%) had lost exon sequences from the mRNA. The remaining mutations were 20% small deletions and frameshifts and 28% base substitutions. The type of mutations observed were similar to those seen in unexposed controls. The site distribution of the mutations, however, indicates that some sequence contexts may be more sensitive to radiation mutagenesis than others.
现在可以通过T淋巴细胞克隆试验直接在人体中检测各种环境因素和治疗药物的致突变影响。我们之前报道过，与未接受治疗的对照组相比，接受放射免疫球蛋白治疗后的癌症患者，其hprt基因座的突变频率增加，且大型基因间缺失的发生率也有所增加。在此，我们报告了对9名接受放射免疫球蛋白治疗的癌症患者中26个独立的hprt突变进行分析的结果。大多数突变（52%）导致mRNA外显子序列缺失。其余突变包括20%的小缺失和移码突变以及28%的碱基替换。观察到的突变类型与未接受治疗的对照组相似。然而，突变的位点分布表明，某些序列背景可能比其他序列背景对辐射诱变更敏感。
