Klinck R, Liquier J, Taillandier E, Gouyette C, Huynh-Dinh T, Guittet E
Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Eur J Biochem. 1995 Oct 15;233(2):544-53. doi: 10.1111/j.1432-1033.1995.544_2.x.
A chemically synthesized 29-base RNA oligomer, designed to fold to form an intramolecular triple helix at acid pH, has been studied by NMR spectroscopy. The molecule consisted of seven U.A.U or C+.G.C base triples joined by two pyrimidine tetra-loops. The fold was such that the third strand was Hoogsteen base-paired in the major groove of a Watson-Crick paired double helix. The nature and size of the molecule required the use of an assignment strategy using two- and three-dimensional homonuclear methods, complemented by a natural abundance 13C correlation experiment. The assignment of the majority of the exchangeable and non-exchangeable resonances is presented. The data suggest a C3'-endo sugar puckering for all the nucleotides involved in base triples. A preliminary structural model consistent with the NMR data is presented.
通过核磁共振光谱法研究了一种化学合成的29个碱基的RNA寡聚物，该寡聚物设计为在酸性pH下折叠形成分子内三螺旋。该分子由七个U.A.U或C⁺.G.C碱基三联体通过两个嘧啶四环连接而成。其折叠方式使得第三条链在沃森-克里克配对双螺旋的大沟中通过Hoogsteen碱基配对。该分子的性质和大小要求使用二维和三维同核方法的归属策略，并辅以天然丰度¹³C相关实验。给出了大多数可交换和不可交换共振的归属。数据表明参与碱基三联体的所有核苷酸均为C3'-内型糖折叠。给出了一个与核磁共振数据一致的初步结构模型。
