Le Dréan E, Gervois N, Diez E, Semana G, Dreno B, Jotereau F
Unité INSERM 211, Nantes, France.
Eur J Immunol. 1995 Oct;25(10):2732-6. doi: 10.1002/eji.1830251003.
CD4+ T cell clones derived from lymphocytes infiltrating four human melanomas specifically recognized melanoma-derived tumor epitopes as shown by secretion of tumor necrosis factor (TNF) in vitro upon interaction with autologous melanoma cells, whereas they did not recognize HLA class II-expressing autologous lymphoblasts or HLA class II mismatched allogeneic melanoma cells. Specificity was further established by demonstrating that TNF responses to tumor cells were inhibited by HLA-DR or HLA-DQ monoclonal antibodies. Most of these clones cross-reacted with allogeneic melanoma cells expressing a potentially restricting HLA allele or a structurally similar one. These data show that shared epitopes of human melanoma cells presented on HLA class II molecules are frequently recognized by autologous CD4+ T lymphocytes.
从浸润4例人类黑色素瘤的淋巴细胞中获得的CD4 + T细胞克隆，在体外与自体黑色素瘤细胞相互作用时，通过分泌肿瘤坏死因子（TNF）显示出对黑色素瘤衍生的肿瘤表位具有特异性识别能力，而它们不识别表达HLA - II类分子的自体淋巴母细胞或HLA - II类错配的同种异体黑色素瘤细胞。通过证明TNF对肿瘤细胞的反应被HLA - DR或HLA - DQ单克隆抗体抑制，进一步确定了特异性。这些克隆中的大多数与表达潜在限制性HLA等位基因或结构相似等位基因的同种异体黑色素瘤细胞发生交叉反应。这些数据表明，HLA - II类分子上呈递的人类黑色素瘤细胞的共享表位经常被自体CD4 + T淋巴细胞识别。
