Vermot-Desroches C, Wijdenes J, Valmu L, Roy C, Pigott R, Nortamo P, Gahmberg C G
Department of Biosciences, University of Helsinki, Finland.
Eur J Immunol. 1995 Sep;25(9):2460-4. doi: 10.1002/eji.1830250908.
We have made a monoclonal anti-CD44 antibody which is able to activate the leukocyte integrin CD11a/CD18. Activated T cells strongly aggregated, and the aggregation was shown to be intercellular adhesion molecule (ICAM)-1 (CD54) and ICAM-2 (CD102) dependent. Using purified ICAM coated on plastic, only binding to ICAM-1 was increased by the CD44 antibody, whereas activation by phorbol ester increased binding to both ICAM-1 and ICAM-3. The binding to ICAM-2 was not affected by either treatment. These findings show that the CD11a/CD18 integrin can be activated in a ligand-specific manner by engagement of CD44.
我们制备了一种单克隆抗CD44抗体，它能够激活白细胞整合素CD11a/CD18。活化的T细胞强烈聚集，且这种聚集被证明依赖细胞间黏附分子（ICAM）-1（CD54）和ICAM-2（CD102）。使用包被在塑料上的纯化ICAM，CD44抗体仅增加与ICAM-1的结合，而佛波酯激活则增加与ICAM-1和ICAM-3两者的结合。两种处理对与ICAM-2的结合均无影响。这些发现表明，CD44的结合可通过配体特异性方式激活CD11a/CD18整合素。
