胰腺炎相关腹水诱导Madin-Darby犬肾细胞发生凋亡性细胞死亡。

Induction of apoptotic cell death by pancreatitis-associated ascitic fluid in Madin-Darby canine kidney cells.

作者信息

Nishikawa J, Takeyama Y, Ueda T, Hori Y, Ueno N, Yamamoto M, Saitoh Y

机构信息

First Department of Surgery, Kobe University School of Medicine, Japan.

出版信息

FEBS Lett. 1995 Oct 2;373(1):19-22. doi: 10.1016/0014-5793(95)00999-p.

DOI:10.1016/0014-5793(95)00999-p

PMID:7589425

Abstract

We investigated the cytotoxicity on Madin-Darby canine kidney (MDCK) cells of pancreatitis-associated ascitic fluid (PAAF) collected from rats with experimental necrotizing pancreatitis. PAAF reduced viability of MDCK cells in a time- and dose-dependent manner. We detected DNA fragmentation on the PAAF-treated MDCK cells, indicating that the cytocidal action of PAAF is via apoptosis. From the results obtained, we conclude that PAAF contains factor(s) inducing apoptosis on MDCK cells, and we assume that apoptotic cell death is involved in the mechanism of organ failure in acute pancreatitis.

摘要

我们研究了从患有实验性坏死性胰腺炎的大鼠体内收集的胰腺炎相关性腹水（PAAF）对犬肾传代细胞（MDCK）的细胞毒性。PAAF以时间和剂量依赖性方式降低了MDCK细胞的活力。我们在经PAAF处理的MDCK细胞上检测到DNA片段化，表明PAAF的杀细胞作用是通过凋亡实现的。根据所得结果，我们得出结论，PAAF含有诱导MDCK细胞凋亡的因子，并且我们推测凋亡性细胞死亡参与了急性胰腺炎器官衰竭的机制。