大鼠棕色和白色脂肪组织中肥胖基因表达的调节。

Modulation of obese gene expression in rat brown and white adipose tissues.

作者信息

Moinat M, Deng C, Muzzin P, Assimacopoulos-Jeannet F, Seydoux J, Dulloo A G, Giacobino J P

机构信息

Département de Biochimie Médicale, Centre Médical Universitaire, Genève, Switzerland.

出版信息

FEBS Lett. 1995 Oct 9;373(2):131-4. doi: 10.1016/0014-5793(95)01030-i.

DOI:10.1016/0014-5793(95)01030-i

PMID:7589451

Abstract

The ob gene mRNA expression in rat brown adipose tissue (BAT) and epididymal white adipose tissue (WAT) was measured on Northern blots hybridized with a rat ob gene probe. The level of ob gene mRNA in BAT was about 40% of that in WAT. Fasting (36 h) or semi-starvation (10 days) decreased the ob gene mRNA level in both tissues by 62-68%, and cold exposure at 6 degrees C (24 h) decreased it in BAT (-84%) but not in WAT. Acute administration of the beta 3-adrenergic agonist Ro 16-8714 decreased the ob gene mRNA level in BAT (-51%) and WAT (-28%) of lean Zucker rats and only in BAT (-74%) of obese falfa rats. This study demonstrates that, in the rat, the ob gene is not only expressed in WAT but also in BAT, and suggests that in these two tissues, the modulation of the ob gene expression might be more closely associated with known alterations in cell lipid content than with changes in sympathetic activity.

摘要

用大鼠ob基因探针进行Northern杂交，测定大鼠棕色脂肪组织（BAT）和附睾白色脂肪组织（WAT）中ob基因mRNA的表达。BAT中ob基因mRNA水平约为WAT中的40%。禁食（36小时）或半饥饿（10天）使两种组织中的ob基因mRNA水平降低62 - 68%，6℃冷暴露（24小时）使BAT中的ob基因mRNA水平降低（-84%），但WAT中未降低。急性给予β3 - 肾上腺素能激动剂Ro 16 - 8714可使瘦型Zucker大鼠的BAT（-51%）和WAT（-28%）以及肥胖型fa/fa大鼠的BAT（-74%）中的ob基因mRNA水平降低。本研究表明，在大鼠中，ob基因不仅在WAT中表达，也在BAT中表达，提示在这两种组织中，ob基因表达的调节可能与细胞脂质含量的已知改变关系更密切，而非与交感神经活性的变化相关。