Nagao M, Morishita E, Hanai Y, Kobayashi K, Sasaki R
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
FEBS Lett. 1995 Oct 16;373(3):225-8. doi: 10.1016/0014-5793(95)01046-h.
Erythropoietin receptor (EPOR) contains a single N-linked sugar in an extracellular domain. It has been suggested that an erythroleukemia cell line with high sensitivity to EPO expresses a high molecular mass form of EPOR, which appears to be a highly N-glycosylated form responsible for EPO-mediated signal transduction [Sawyer and Hankins (1993) Proc. Natl. Acad. Sci. USA 90, 6849-6853]. To examine the role of the N-linked sugar chain, we prepared EPO-dependent cell lines expressing the wild-type EPOR and N-glycosylation-defective EPOR. There was little difference in the expression of EPOR on the cell surface, EPO binding kinetics, and EPO-induced cell proliferation between the clones expressing the mutant EPOR and those expressing the wild-type EPOR.
促红细胞生成素受体（EPOR）在细胞外结构域含有一个单一的N-连接糖。有人提出，对促红细胞生成素高度敏感的红白血病细胞系表达高分子量形式的EPOR，这似乎是一种高度N-糖基化形式，负责促红细胞生成素介导的信号转导[索耶尔和汉金斯（1993年）《美国国家科学院院刊》90，6849 - 6853]。为了研究N-连接糖链的作用，我们制备了表达野生型EPOR和N-糖基化缺陷型EPOR的促红细胞生成素依赖性细胞系。在表达突变型EPOR的克隆和表达野生型EPOR的克隆之间，细胞表面EPOR的表达、促红细胞生成素结合动力学以及促红细胞生成素诱导的细胞增殖几乎没有差异。
