衰老过程中铜锌超氧化物歧化酶组氨酸残基的变化。

Changes in the histidine residues of Cu/Zn superoxide dismutase during aging.

作者信息

Maria C S, Revilla E, Ayala A, de la Cruz C P, Machado A

机构信息

Departamento de Bioquimica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, Spain.

出版信息

FEBS Lett. 1995 Oct 23;374(1):85-8. doi: 10.1016/0014-5793(95)01083-q.

DOI:10.1016/0014-5793(95)01083-q

PMID:7589519

Abstract

Cu/Zn-Superoxide dismutase activity (Cu/Zn-SOD) was studied in liver from 3- and 24-month-old rat. A significant decrease of enzyme activity in liver of the aged rat was found. Various amino acid residues and protein carbonyl groups (CO) were measured in purified young and old enzyme. It was found that the 'old' enzyme had one histidine fewer and higher CO content than the 'young' Cu/Zn-SOD. Inactivation 'in vitro' of purified commercial bovine erythrocyte Cu/Zn-SOD led to a decrease in the enzymatic activity, an increase in the CO and one histidine residue modified. A similar behavior between aging and oxidation was suggested.

摘要

对3月龄和24月龄大鼠肝脏中的铜锌超氧化物歧化酶活性（Cu/Zn-SOD）进行了研究。发现老年大鼠肝脏中的酶活性显著降低。对纯化后的年轻和老年酶中的各种氨基酸残基和蛋白质羰基（CO）进行了测定。结果发现，“老年”酶比“年轻”的铜锌超氧化物歧化酶少一个组氨酸且羰基含量更高。纯化的商业牛红细胞铜锌超氧化物歧化酶在“体外”失活导致酶活性降低、羰基增加且一个组氨酸残基被修饰。由此表明衰老和氧化之间存在相似的行为。