Neuman K, Nornes H O, Neuman T
Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523, USA.
FEBS Lett. 1995 Oct 30;374(2):279-83. doi: 10.1016/0014-5793(95)01128-2.
Id-like helix-loop-helix (HLH) transcription factors are involved in the regulation of proliferation and differentiation of several cell types. We isolated 5' regulatory region of mouse Id2 gene and demonstrated that it contains several E-box clusters. These E-boxes mediate stimulatory effects of basic-HLH (bHLH) transcription factors ME1, ME2, and NSCL1 on Id2 promoter activity. Co-expression of Id2 blocks the stimulatory effect of bHLH transcription factors which suggests the presence of feedback loops in Id2 transcriptional regulation. Overexpression of NSCL1 in F9 cells blocks the downregulation of Id2 gene expression during retinoic acid induced differentiation. Our data demonstrate that bHLH transcription factors regulate Id2 gene expression.
类 Id 螺旋-环-螺旋（HLH）转录因子参与多种细胞类型的增殖和分化调控。我们分离了小鼠 Id2 基因的 5'调控区，并证明其包含多个 E 盒簇。这些 E 盒介导碱性-HLH（bHLH）转录因子 ME1、ME2 和 NSCL1 对 Id2 启动子活性的刺激作用。Id2 的共表达阻断了 bHLH 转录因子的刺激作用，这表明在 Id2 转录调控中存在反馈环。NSCL1 在 F9 细胞中的过表达阻断了维甲酸诱导分化过程中 Id2 基因表达的下调。我们的数据表明 bHLH 转录因子调控 Id2 基因表达。
