Paschen W, Patscheke H, Wörner P
Blut. 1979 Jan 22;38(1):17-24. doi: 10.1007/BF01082924.
Walker 256 carcinoma cells form irreversible aggregates with rat platelets activated by ADP or serotonin. Since serotonin induces platelet shape change but not platelet aggregation the degree of activation indicated by the disc-sphere transformation is sufficient for platelets to interact with these tumor cells. This is confirmed by experiments with spheroid washed platelets which form irreversible mixed aggregates with Walker 256 carcinoma cells without a stimulus being required. This type of tumor cells could react with platelets in vivo, provided the platelets are activated by disturbed blood flow or contact with subendothelium. Our observations can explain why other authors found no interaction between Walker 256 carcinoma cells and non-activated platelets in vitro even though platelets contributed to the formation of bloodborne metastases of this tumor.
Walker 256癌细胞与由ADP或血清素激活的大鼠血小板形成不可逆聚集体。由于血清素可诱导血小板形态改变但不诱导血小板聚集，因此由圆盘 - 球体转变所表明的激活程度足以使血小板与这些肿瘤细胞相互作用。这一点通过用球形洗涤血小板进行的实验得到证实，这些血小板与Walker 256癌细胞形成不可逆的混合聚集体，无需刺激。如果血小板因血流紊乱或与内皮下层接触而被激活，这种类型的肿瘤细胞可在体内与血小板发生反应。我们的观察结果可以解释为什么其他作者在体外未发现Walker 256癌细胞与未激活的血小板之间存在相互作用，尽管血小板促成了该肿瘤血行转移的形成。
