卵胞浆内单精子注射对受精失败卵母细胞进行再受精的前瞻性自身对照研究。

Prospective, auto-controlled study on reinsemination of failed-fertilized oocytes by intracytoplasmic sperm injection.

作者信息

Nagy Z P, Staessen C, Liu J, Joris H, Devroey P, Van Steirteghem A C

机构信息

Centre for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Belgium.

出版信息

Fertil Steril. 1995 Dec;64(6):1130-5.

PMID:7589665

Abstract

OBJECTIVE

To evaluate the possible influence of late fertilization after standard IVF on the results of reinsemination of assumed failed-fertilized oocytes by microinjection and to examine the correlation between the effect of aging of (failed-fertilized) oocytes and the ability of these oocytes to become fertilized.

DESIGN

Trial 1: Group 1 (injected-day 1), 93 failed-fertilized oocytes injected 1 day after ovum pick-up; group 2 (control), 82 failed-fertilized oocytes with no microinjection performed. Trial 2: Group 1 (ICSI-day 1), 40 failed-fertilized oocytes injected 1 day after ovum pick-up; group-2 (ICSI-day 2), 40 failed-fertilized oocytes injected 2 days after ovum pick-up. In addition, 35 two- to eight-cell stage embryos, obtained after ICSI of IVF failed-fertilized oocytes, were fixed for cytogenetic analysis.

MAIN OUTCOME MEASURES

Normal and abnormal fertilization and embryo development.

RESULTS

Trial 1: 53% normal (2 pronuclear [PN]) and 25% abnormal (> or = 3PN) fertilization rates were obtained in group 1 (injected-day 1), and 71% of the 2PN and 74% of the > or = 3PN oocytes cleaved with < 50% fragmentation. No pronuclear (> or = 2PN) development occurred in the control group. Trial 2: 45% and 8% normal and 25% and 40% abnormal fertilization rates were obtained, respectively, after ICSI of 1-day-old and 2-day-old failed-fertilized oocytes. Two days after microinjection, 67% and 67% of the 2PN and 80% and 44% of the > or = 3PN oocytes cleaved with < 50% fragmentation in group ICSI-day 1 and in group ICSI-day 2, respectively.

CONCLUSIONS

Late fertilization after initial in vitro insemination does not play a role in the high fertilization rate obtained after reinsemination of assumed failed-fertilized oocytes by ICSI. Normal (2PN) fertilization rate, however, decreases strongly and the abnormal (> or = 3PN) fertilization rate increases with oocyte aging and derived embryos seem to have a high incidence of cytogenetic abnormalities.

摘要

目的

评估标准体外受精（IVF）后延迟受精对假定受精失败的卵母细胞经显微注射重新受精结果的可能影响，并研究（受精失败的）卵母细胞老化效应与这些卵母细胞受精能力之间的相关性。

设计

试验1：第1组（注射日1），93个受精失败的卵母细胞在取卵后1天进行注射；第2组（对照组），82个受精失败的卵母细胞未进行显微注射。试验2：第1组（卵胞浆内单精子注射日1），40个受精失败的卵母细胞在取卵后1天进行注射；第2组（卵胞浆内单精子注射日2），40个受精失败的卵母细胞在取卵后2天进行注射。此外，对IVF受精失败的卵母细胞经卵胞浆内单精子注射后获得的35个二至八细胞期胚胎进行固定，用于细胞遗传学分析。

主要观察指标

正常和异常受精及胚胎发育情况。

结果

试验1：第1组（注射日1）正常（2原核[PN]）受精率为53%，异常（≥3PN）受精率为25%，2PN卵母细胞中有71%、≥3PN卵母细胞中有74%发生卵裂，碎片率<50%。对照组未出现原核（≥2PN）发育。试验2：1日龄和2日龄受精失败的卵母细胞经卵胞浆内单精子注射后，正常受精率分别为45%和8%，异常受精率分别为25%和40%。显微注射2天后，卵胞浆内单精子注射日1组和卵胞浆内单精子注射日2组中，2PN卵母细胞的卵裂率分别为67%和67%，≥3PN卵母细胞的卵裂率分别为80%和44%，碎片率<50%。