Chu I, Villeneuve D C, Yagminas A, Lecavalier P, Håkansson H, Ahlborg U G, Valli V E, Kennedy S W, Bergman A, Seegal R F
Environmental Health Directorate and Food Directorate, Health Protection Branch, Ottawa, Ontario, Canada.
Fundam Appl Toxicol. 1995 Jul;26(2):282-92. doi: 10.1006/faat.1995.1099.
The toxicity of 3,3',4,4'-tetrachlorobiphenyl (PCB 77) and 2,3',4,4',5-pentachlorobiphenyl (PCB 118) was investigated in rats following subchronic dietary exposure. Groups of 10 male and 10 female weanling Sprague-Dawley rats were administered PCB 77 in the diet at 0, 10, 100, 1000, or 10,000 ppb for 13 weeks. PCB 118 was administered to males in the diet at 0, 10, 100, 1000, and 10,000 ppb, while the female groups received 0, 2, 20, 200, or 2000 ppb of the congener for 13 weeks. Growth rate and food consumption were not affected by treatment. No clinical signs of toxicity were observed. Increased spleen weight occurred in male rats fed 1000 or 10,000 ppb PCB 77. Male rats receiving 10,000 ppb PCB 118 had increased liver weight and hepatic ethoxyresorufin O-deethylase (EROD) activity. Increased hepatic EROD activity but not liver weight was observed in female rats given the 2000-ppb PCB 118 diet. Increased EROD activity was also noted in male rats given 10,000 ppb and in female groups receiving 1000 or 10,000 ppb PCB 77. Male rats exposed to 10,000 ppb PCB 77 had decreased vitamin A in the liver and lung and elevated levels in the kidney. Liver vitamin A of both 1000- and 10,000-ppb PCB 77 female groups was decreased. PCB 118 had no effects on tissue vitamin A at the levels studied. No hematological changes or serum biochemical changes were seen in any of PCB 118- and PCB 77-treated groups, nor were liver uroporphyrin levels altered. A reduction in dopamine and homovanillinic acid in substantia nigra region of the brain was observed in female rats fed 2000 ppb PCB 118, while 10,000 ppb PCB 77 was associated with an elevation in 3,4-dihydroxyphenylacetic acid in the nucleus accumbens region of male rat brains. Mild to moderate changes were observed in the liver and thyroid of rats given PCB 77 or PCB 118. PCB 118 accumulated in a dose-dependent manner in fat and to a much lesser extent in liver. In contrast, very low levels of PCB 77 residue were found in the tissues examined. Based on the above data it was concluded that NOAEL of PCB 77 is 100 ppb in diet or 8.7 micrograms/kg and that of PCB 118 is 200 ppb in diet or 17 micrograms/kg body wt/day.
在大鼠经亚慢性膳食暴露后，对3,3',4,4'-四氯联苯（多氯联苯77，PCB 77）和2,3',4,4',5-五氯联苯（多氯联苯118，PCB 118）的毒性进行了研究。将10只雄性和10只雌性断乳的斯普拉格-道利大鼠分为若干组，分别给予含0、10、100、1000或10,000 ppb的PCB 77的饲料，持续13周。给予雄性大鼠含0、10、100、1000和10,000 ppb的PCB 118的饲料，而雌性大鼠组则给予含0、2、20、200或2000 ppb该同系物的饲料，持续13周。生长速率和食物消耗量未受处理影响。未观察到毒性的临床体征。饲喂1000或10,000 ppb PCB 77的雄性大鼠脾脏重量增加。接受10,000 ppb PCB 118的雄性大鼠肝脏重量增加，肝乙氧基异吩唑酮O-脱乙基酶（EROD）活性增强。给予2000 ppb PCB 118饲料的雌性大鼠肝脏EROD活性增强，但肝脏重量未增加。给予10,000 ppb的雄性大鼠以及接受1000或10,000 ppb PCB 77的雌性大鼠组中也观察到EROD活性增强。暴露于10,000 ppb PCB 77的雄性大鼠肝脏和肺中的维生素A含量降低，而肾脏中的含量升高。1000和10,000 ppb PCB 77雌性组的肝脏维生素A均降低。在所研究的剂量水平下，PCB 118对组织维生素A没有影响。在任何PCB 118和PCB 77处理组中均未观察到血液学变化或血清生化变化，肝脏尿卟啉水平也未改变。饲喂2000 ppb PCB 118的雌性大鼠大脑黑质区域的多巴胺和高香草酸减少，而10,000 ppb PCB 77与雄性大鼠大脑伏隔核区域的3,4-二羟基苯乙酸升高有关。在给予PCB 77或PCB 118的大鼠的肝脏和甲状腺中观察到轻度至中度变化。PCB 118以剂量依赖性方式在脂肪中蓄积，在肝脏中的蓄积程度要小得多。相比之下，在所检查的组织中发现PCB 77残留水平非常低。根据上述数据得出结论，PCB 77的无观察到有害作用水平（NOAEL）为饲料中100 ppb或8.7微克/千克，PCB 118的为饲料中200 ppb或17微克/千克体重/天。
