Klimisch H J, Hellwig J
Department of Toxicology, BASF Aktiengesellschaft, Ludwigshafen, Germany.
Fundam Appl Toxicol. 1995 Aug;27(1):77-89. doi: 10.1006/faat.1995.1110.
3-Methyl-1-butanol (MEB) and 2-methyl-1-propanol (MEP) were tested for their prenatal inhalation toxicity in pregnant Wistar rats or Himalayan rabbits. Twenty-five female rats and 15 female rabbits per group were exposed to MEB and MEP vapors at concentrations of 10, 2.5, or 0.5 mg/liter, 6 hr/day. The rats were exposed on Days 6-15 postcoitum (pc) and the rabbits were exposed on Days 7-19 postinsemination (pi). Control groups were exposed to clean air. The body weights of the animals of either species were determined several times throughout the studies. All rats and all rabbits were killed on Day 20 pc and Day 29 pi, respectively. The fetuses were removed from the uterus and examined for compound-related effects. The high concentration of 10 mg/liter caused a slight retardation of body weight gain in the dams of either species exposed to MEB and in the dams of rabbits exposed to MEP during the first days of the exposure period. Eye irritation was observed only in the MEB-treated rabbits during the period of exposure to 10 mg/liter. The fetuses of either species exhibited no signs of embryo-/fetotoxicity or teratogenic effects caused by MEP or MEB. Under the experimental conditions, 2.5 mg/liter was found to be a no-observable-adverse-effect level (NOAEL) for the dams of either species exposed to MEB and for the does exposed to MEP, whereas 10 mg/liter MEP was the NOAEL for the maternal rats. For both substances 10 mg/liter was defined as the NOAEL for the conceptuses of either species.
对3-甲基-1-丁醇（MEB）和2-甲基-1-丙醇（MEP）进行了孕期吸入毒性测试，实验对象为怀孕的Wistar大鼠或喜马拉雅兔。每组25只雌性大鼠和15只雌性兔子，每天6小时暴露于浓度为10、2.5或0.5毫克/升的MEB和MEP蒸汽中。大鼠在交配后第6至15天（pc）进行暴露，兔子在授精后第7至19天（pi）进行暴露。对照组暴露于清洁空气中。在整个研究过程中多次测定两种动物的体重。所有大鼠和兔子分别在怀孕第20天和授精后第29天处死。将胎儿从子宫中取出，检查与化合物相关的影响。在暴露期的头几天，10毫克/升的高浓度导致暴露于MEB的两种动物的母鼠以及暴露于MEP的兔子母鼠体重增加略有迟缓。仅在暴露于10毫克/升的MEB处理的兔子中观察到眼部刺激。两种动物的胎儿均未表现出由MEP或MEB引起的胚胎/胎儿毒性或致畸作用迹象。在实验条件下，发现2.5毫克/升是暴露于MEB的两种动物的母鼠以及暴露于MEP的母兔的无可见不良作用水平（NOAEL），而10毫克/升的MEP是母鼠的NOAEL。对于两种物质，10毫克/升被定义为两种动物的胚胎的NOAEL。
