Kippert F, Lloyd D
School of Pure and Applied Biology, University of Wales College of Cardiff, UK.
FEMS Microbiol Lett. 1995 Oct 15;132(3):215-9. doi: 10.1111/j.1574-6968.1995.tb07836.x.
A novel method is described which uses aniline blue for the specific fluorescent staining of the septa of dividing cells of the fission yeast, Schizosaccharomyces pombe. It gives the same results with live and fixed cells. In fixed or, more generally, dead cells there is no staining of the cytoplasm: this renders aniline blue superior to other dyes previously used to stain the septum of S. pombe. This feature allows quantitative analysis of the septum index for fixed samples and, therefore, makes aniline blue the stain of choice for cell cycle kinetic studies.
本文描述了一种新方法，该方法使用苯胺蓝对裂殖酵母（Schizosaccharomyces pombe）分裂细胞的隔膜进行特异性荧光染色。该方法对活细胞和固定细胞均能得到相同的结果。在固定细胞或更普遍的死细胞中，细胞质不会被染色：这使得苯胺蓝优于先前用于染色粟酒裂殖酵母隔膜的其他染料。这一特性允许对固定样本的隔膜指数进行定量分析，因此，使苯胺蓝成为细胞周期动力学研究的首选染色剂。
