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The use of primuline to identify the septum polysaccharide of the fission yeast Schizosaccharomyces pombe.

作者信息

Duffus J H, McDowell W, Manners D J

出版信息

Stain Technol. 1984 Mar;59(2):79-82. doi: 10.3109/10520298409113836.

DOI:10.3109/10520298409113836
PMID:6207634
Abstract

Treatment of cells and purified cell walls of the fission yeast Schizosaccharomyces pombe with primuline reveals the septum as a bright fluorescent band. When polysaccharides containing (1----3)-beta-, (1----6)-beta- or (1----3)-alpha-glucosidic linkages are treated with primuline, only those molecules containing chains of (1----3)-beta-glucosyl residues are stained. This implies that (1----3)-beta-glucan is present in the septum of Schiz. pombe as the main constituent.

摘要

相似文献

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The use of primuline to identify the septum polysaccharide of the fission yeast Schizosaccharomyces pombe.
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