Davis L G, Sass N L, Manna B, Nusynowitz M L
Clin Chem. 1979 Feb;25(2):218-20.
A sensitivie, reliable gas-chromatographic assay for monoiodotyrosine and diiodotyrosine in human serum is reported. The oxazolidinone-heptafluorobutyric anhydride derivatives allow the quantitation of both compounds in the linear range of 0.2 to 7.6 mg/L of serum. Analytical recovery averaged 88%, and mean accuracy and within-run precision were 98 and 2%, respectively. Concentrations of monoiodotyrosine in serum as low as 20 microgram/L and of diiodotyrosine as low as 100 microgram/L can be detected. Normal serum contains no detectable concentration of either compound, but the method is applicable as a diagnostic tool in the early prediction of thyroid disease. Both compounds were detected in the serum of a hypothyroid subject whose normal thyroid hormone concentrations were being maintained by therapy with desiccated thyroid extract.
本文报道了一种灵敏、可靠的用于测定人血清中单碘酪氨酸和二碘酪氨酸的气相色谱分析法。恶唑烷酮-七氟丁酸酐衍生物可在血清浓度0.2至7.6mg/L的线性范围内对这两种化合物进行定量。分析回收率平均为88%，平均准确度和批内精密度分别为98%和2%。血清中单碘酪氨酸的检测下限低至20μg/L，二碘酪氨酸的检测下限低至100μg/L。正常血清中未检测到这两种化合物的任何浓度，但该方法可作为甲状腺疾病早期预测的诊断工具。在一名甲状腺功能减退患者的血清中检测到了这两种化合物，该患者通过服用干燥甲状腺提取物进行治疗，甲状腺激素浓度维持在正常水平。
