Flink I L, Morkin E
University Heart Center, Tucson, AZ 85724, USA.
Gene. 1995 Oct 3;163(2):279-82. doi: 10.1016/0378-1119(95)00421-2.
The human cellular CNBP gene locus has been sequenced and is comprised of 6453 bp from the transcription start point (tsp) to the polyadenylation signal, and an additional 201 bp of 5' and 259 bp of 3' flanking sequences. The gene consists of five exons, four of which contain coding information for two alternatively spliced products, CNPB alpha and beta. The open reading frame (ORF) of 177 amino acids (aa) is encoded by exons 2 through 4 (CNBP alpha, M(r) 19463). The protein contains seven zinc-finger (Zf) domains. CNBP beta lacks seven aa in the linker region between the first two Zf, because of the use of an alternative 5' donor site within exon 2. The sixth Zf is the only Zf domain that is not completely encoded within a single exon and is interrupted by an intron (intron 4). The 5' untranslated region (UTR) contains 849 bp and is interrupted by intron 1. The 3' UTR, ending at the polyadenylation signal, is 857 bp long and is contained within exon 5. One Alu repeat was identified within intron 1, the largest intron of CNBP.
人类细胞中的CNBP基因座已被测序，从转录起始点（tsp）到聚腺苷酸化信号由6453 bp组成，还有额外的5'端201 bp和3'端侧翼序列259 bp。该基因由五个外显子组成，其中四个外显子包含两种可变剪接产物CNPBα和β的编码信息。177个氨基酸（aa）的开放阅读框（ORF）由外显子2至4编码（CNBPα，M（r）19463）。该蛋白质包含七个锌指（Zf）结构域。由于在外显子2内使用了一个替代的5'供体位点，CNBPβ在前两个Zf之间的连接区域缺少七个氨基酸。第六个Zf是唯一不完全在单个外显子内编码且被一个内含子（内含子4）中断的Zf结构域。5'非翻译区（UTR）包含849 bp，并被内含子1中断。3'UTR在聚腺苷酸化信号处结束，长857 bp，包含在外显子5内。在内含子1（CNBP最大的内含子）中鉴定出一个Alu重复序列。
