兔甘油醛-3-磷酸脱氢酶编码cDNA的序列
Sequence of the rabbit glyceraldehyde-3-phosphate dehydrogenase-encoding cDNA.
作者信息
Applequist S E, Keyna U, Calvin M R, Beck-Engeser G B, Raman C, Jäck H M
机构信息
Stritch School of Medicine, Loyola University of Chicago, Maywood, IL 60153, USA.
出版信息
Gene. 1995 Oct 3;163(2):325-6. doi: 10.1016/0378-1119(95)00386-k.
Two full-length cDNA clones encoding rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were isolated from a lambda gt10 rabbit spleen cDNA library and sequenced. As predicted from the open reading frame (ORF) in vitro translation of a sense orientation GAPDH cDNA clone yielded a protein product with a molecular mass of 37 kDa. Rabbit GAPDH exhibits a high degree of homology to the mouse, rat, hamster, chicken and human GAPDH on both the nucleotide (nt) and amino acid (aa) levels.
从λgt10兔脾脏cDNA文库中分离出两个编码兔甘油醛-3-磷酸脱氢酶（GAPDH）的全长cDNA克隆并进行测序。正如从开放阅读框（ORF）预测的那样，正义方向的GAPDH cDNA克隆的体外翻译产生了一种分子量为37 kDa的蛋白质产物。兔GAPDH在核苷酸（nt）和氨基酸（aa）水平上与小鼠、大鼠、仓鼠、鸡和人GAPDH都具有高度同源性。
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