编码人精氨酰 - tRNA合成酶的cDNA的克隆与鉴定

Cloning and characterization of cDNA encoding a human arginyl-tRNA synthetase.

作者信息

Girjes A A, Hobson K, Chen P, Lavin M F

机构信息

Queensland Cancer Fund Research Unit, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.

出版信息

Gene. 1995 Oct 27;164(2):347-50. doi: 10.1016/0378-1119(95)00502-w.

DOI:10.1016/0378-1119(95)00502-w

PMID:7590355

Abstract

Arginyl-tRNA synthetase (ArgRS) plays a key role in protein synthesis as part of a multienzyme complex with a number of other aminoacyl-tRNA synthetase (aaRS) enzymes. We have isolated a full-length cDNA encoding ArgRS as part of a project on complementation of radiosensitivity in human cells with an Epstein-Barr Virus (EBV) vector-based human cDNA library. DNA sequence analysis identified an open reading frame of 1983 nucleotides with 87% homology to other mammalian ArgRS genes. The deduced amino acid (aa) sequence (661 aa) showed 87.7% identity to the Chinese hamster ovary (CHO) enzyme and 37.7% identity to the homologous Escherichia coli enzyme. Northern blot analysis revealed the presence of a single mRNA species of approx. 2.2 kb. The results described here demonstrate that ArgRS is highly conserved in mammalian cells and confirm the presence of a hydrophobic N-terminal region in the higher-molecular-weight complexed form of ArgRS.

摘要

精氨酰 - tRNA合成酶（ArgRS）作为与许多其他氨酰 - tRNA合成酶（aaRS）酶组成的多酶复合物的一部分，在蛋白质合成中起关键作用。作为一个用基于爱泼斯坦 - 巴尔病毒（EBV）载体的人类cDNA文库互补人类细胞放射敏感性项目的一部分，我们分离出了编码ArgRS的全长cDNA。DNA序列分析鉴定出一个1983个核苷酸的开放阅读框，与其他哺乳动物ArgRS基因有87%的同源性。推导的氨基酸（aa）序列（661个aa）与中国仓鼠卵巢（CHO）酶有87.7%的同一性，与同源大肠杆菌酶有37.7%的同一性。Northern印迹分析显示存在一种约2.2 kb的单一mRNA种类。此处描述的结果表明ArgRS在哺乳动物细胞中高度保守，并证实了在较高分子量复合形式的ArgRS中存在一个疏水的N端区域。