促甲状腺激素释放激素对离体大鼠胰腺腺泡细胞酶分泌的抑制作用。

Inhibitory effect of thyrotropin-releasing hormone on enzyme secretion from isolated rat pancreatic acinar cells.

作者信息

Kemmer T P, Duntas L, Munz W, Malfertheiner P

机构信息

Rehabilitationsklinik Neresheim, Germany.

出版信息

Horm Metab Res. 1995 Aug;27(8):367-71. doi: 10.1055/s-2007-979980.

DOI:10.1055/s-2007-979980

PMID:7590625

Abstract

This study reports a direct effect of TRH on amylase secretion from isolated rat exocrine pancreatic acinar cells. TRH inhibited carbachol (10(-5) M)-stimulated amylase secretion by a maximum of 24% at a concentration of 10(-11) M (p < 0.05), but did not affect basal amylase release in concentrations from 10(-13) M to 10(-8) M. Ceruletide (3 x 10(-10) M)-stimulated amylase secretion was maximally reduced by 23% at a TRH concentration of 10(-10) M (p < 0.05). Direct stimulation of protein kinase C-mediated secretion by the diacylglycerol analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) was not altered by TRH. The TRH metabolite cyclo (His-Pro) did not influence basal or stimulated pancreatic secretion in vitro. These findings point to a TRH-mediated modulation of exocrine pancreatic secretion at the receptor site.

摘要

本研究报告了促甲状腺激素释放激素（TRH）对分离的大鼠胰腺外分泌腺泡细胞淀粉酶分泌的直接作用。TRH在浓度为10⁻¹¹M时，对卡巴胆碱（10⁻⁵M）刺激的淀粉酶分泌的抑制作用最大可达24%（p<0.05），但在10⁻¹³M至10⁻⁸M的浓度范围内不影响基础淀粉酶释放。在TRH浓度为10⁻¹⁰M时，蛙皮素（3×10⁻¹⁰M）刺激的淀粉酶分泌最大减少23%（p<0.05）。二酰基甘油类似物1-油酰基-2-乙酰基-sn-甘油（OAG）对蛋白激酶C介导的分泌的直接刺激不受TRH影响。TRH代谢产物环（组氨酸-脯氨酸）在体外不影响基础或刺激的胰腺分泌。这些发现表明TRH在受体部位对外分泌性胰腺分泌有介导的调节作用。