Johnson M S, Senger P L, Allen C H, Hancock D D, Alexander B M, Sasser R G
Department of Animal Sciences, Washington State University, Pullman 99164-6332, USA.
J Anim Sci. 1995 Jul;73(7):1914-9. doi: 10.2527/1995.7371914x.
The fertility of bull semen packaged in .25- and .5-mL french straws was compared. One ejaculate from each of five Holstein bulls was split, extended to 10 x 10(6) spermatozoa/inseminate dose in whole homogenized milk, packaged in .25- and .5-mL french straws, frozen in liquid nitrogen (LN) vapor, and stored in LN. Semen was thawed at 37 degrees C for 30 s. Synchronized heifers (n = 1,360) were inseminated (during a 12-mo period) with semen packaged in either a .25- or .5-mL french straw. Blood was collected on the day of insemination and the serum was assayed for progesterone. Heifers with blood progesterone levels of > 1 ng/mL were eliminated from the data. Blood was collected at 30 to 45 d after insemination and the serum was assayed for the presence of bovine pregnancy-specific protein B (bPSPB) by RIA to determine pregnancy. Conception was 63.6 and 62.0% (P = .55) for semen packaged in the .25- and .5-mL french straws, respectively. There was neither a bull x packaging unit interaction (P = .49) nor a day of insemination x packaging unit interaction (P = .87). Conception among bulls ranged from 57.1 to 68.0% (P = .19). No evidence was found that meteorological factors influenced conception. Under the conditions of this experiment, semen packaged in the .25- and .5-mL french straw had similar fertility.
比较了包装在0.25毫升和0.5毫升法式细管中的公牛精液的生育力。从5头荷斯坦公牛中每头采集一份射精量，将其分割，在全脂均质牛奶中稀释至每输精剂量含10×10⁶个精子，分别包装在0.25毫升和0.5毫升法式细管中，在液氮（LN）蒸气中冷冻，并储存在LN中。精液在37℃解冻30秒。在12个月期间，将同期发情的小母牛（n = 1360）用包装在0.25毫升或0.5毫升法式细管中的精液进行输精。在输精当天采集血液，检测血清中的孕酮。血液孕酮水平>1 ng/mL的小母牛被排除在数据之外。在输精后30至45天采集血液，通过放射免疫分析（RIA）检测血清中牛妊娠特异性蛋白B（bPSPB）的存在以确定妊娠情况。包装在0.25毫升和0.5毫升法式细管中的精液的受胎率分别为63.6%和62.0%（P = 0.55）。既没有公牛×包装单位的交互作用（P = 0.49），也没有输精日期×包装单位的交互作用（P = 0.87）。公牛之间的受胎率在57.1%至68.0%之间（P =
