McNeel R L, Mersmann H J
Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA.
J Anim Sci. 1995 Jul;73(7):1962-71. doi: 10.2527/1995.7371962x.
Expression of transcripts for beta 1-, beta 2-, and beta 3-adrenergic receptors (beta 1-AR, beta 2-AR, and beta 3-AR, respectively) was investigated in porcine tissues. Northern blot analysis of total RNA indicated hybridization of the rat beta 1-AR probe to transcripts of approximately 3.0-kb in heart and lung and 2.9-kb in perirenal, omental, and subcutaneous adipose tissue. Hybridization of the hamster beta 2-AR probe with total RNA revealed transcripts of approximately 1.8-kb in the heart, 2.0-kb in the lung, and 2.0-kb in perirenal, omental, and subcutaneous adipose tissue; a minor transcript of 1.8-kb was also observed in the perirenal tissue. Northern blot analysis of poly (A+) RNA from porcine adipose tissue with the mouse beta 3-AR probe revealed no detectable signals in heart and lung but detected a major 2.2-kb transcript and a minor 1.9-kb transcript in subcutaneous adipose tissue. The expression of porcine beta 1-AR and beta 2-AR transcripts was compared in perirenal, omental, and subcutaneous adipose depots. The expression of beta 1-AR transcripts was four to five times greater in the perirenal depot than in the omental depot and two to three times greater in the subcutaneous depot than in the omental depot. The expression of beta 2-AR mRNA seemed to be the same in all three depots.
研究了猪组织中β1-、β2-和β3-肾上腺素能受体（分别为β1-AR、β2-AR和β3-AR）转录本的表达情况。对总RNA进行Northern印迹分析表明，大鼠β1-AR探针与心脏和肺中约3.0 kb以及肾周、网膜和皮下脂肪组织中约2.9 kb的转录本杂交。仓鼠β2-AR探针与总RNA杂交显示，心脏中约1.8 kb、肺中约2.0 kb以及肾周、网膜和皮下脂肪组织中约2.0 kb的转录本；在肾周组织中也观察到一个1.8 kb的次要转录本。用小鼠β3-AR探针对猪脂肪组织的聚腺苷酸（A+）RNA进行Northern印迹分析，在心脏和肺中未检测到可检测信号，但在皮下脂肪组织中检测到一个主要的2.2 kb转录本和一个次要的1.9 kb转录本。比较了猪肾周、网膜和皮下脂肪库中β1-AR和β2-AR转录本的表达。β1-AR转录本在肾周脂肪库中的表达比网膜脂肪库高4至5倍，在皮下脂肪库中的表达比网膜脂肪库高2至3倍。β2-AR mRNA在所有三个脂肪库中的表达似乎相同。
