Balcells J, Seal C J, Parker D S
Department of Biological and Nutritional Sciences, Faculty of Agriculture and Biological Sciences, University of Newcastle upon Tyne, U.K.
J Anim Sci. 1995 Jul;73(7):2146-55. doi: 10.2527/1995.7372146x.
This experiment investigated the effect of intrajugular infusion of glucose on whole-body glucose metabolism and the absorption of nutrients by the portal-drained viscera of four Suffolk-cross sheep, average BW 46 +/- 7 kg, fed a cereal/straw-based pelleted diet. Each sheep received by random allocation 0 (control), 1.0, or 2.0 mg of glucose.kg BW-1.min-1 for 8 h infused into the jugular vein. Glucose irreversible loss rate, measured by simultaneous infusion of 6-[3H]-glucose, increased approximately stoichiometrically during glucose infusion (.51, .75, and 1.09 error mean square [EMS] .032 mmol/min for control, 1.0, and 2.0 mg of glucose infused.kg BW-1.min-1, respectively). Utilization of glucose increased in portal tissues (P = .089) as a result of glucose infusion (.17, .18, and .33 EMS .008 mmol/min) and was a constant proportion of glucose irreversible loss (.28). Portal blood flow was not affected by glucose infusion (overall mean 1.13 L/min EMS .034). Net portal absorption of acetate increased during glucose infusion although ruminal VFA concentrations were not affected. In contrast, net free amino acid absorption by portal-drained tissues was reduced during glucose infusion (291, 115, and 4 EMS 33,816 mumol/min, P = .054). These results show that metabolism of nutrients across the gut wall is influenced by glucose availability to gastrointestinal tissues and affects the pattern of nutrients available to the liver and peripheral tissues.
本实验研究了颈静脉输注葡萄糖对4只萨福克杂交绵羊（平均体重46±7千克，饲喂谷物/秸秆颗粒饲料）全身葡萄糖代谢及门静脉引流内脏对营养物质吸收的影响。每只绵羊通过随机分配接受0（对照）、1.0或2.0毫克葡萄糖·千克体重⁻¹·分钟⁻¹的剂量，经颈静脉输注8小时。通过同时输注6-[³H]-葡萄糖测量的葡萄糖不可逆损失率，在葡萄糖输注期间大致呈化学计量增加（对照、输注1.0和2.0毫克葡萄糖·千克体重⁻¹·分钟⁻¹时，分别为0.51、0.75和1.09误差均方[EMS]0.032毫摩尔/分钟）。由于葡萄糖输注，门静脉组织中葡萄糖的利用率增加（P = 0.089）（分别为0.17、0.18和0.33 EMS 0.008毫摩尔/分钟），且占葡萄糖不可逆损失的恒定比例（0.28）。葡萄糖输注对门静脉血流量无影响（总体平均值1.13升/分钟EMS 0.034）。尽管瘤胃挥发性脂肪酸浓度未受影响，但葡萄糖输注期间门静脉对乙酸盐的净吸收增加。相反，葡萄糖输注期间门静脉引流组织对游离氨基酸的净吸收减少（分别为291、115和4 EMS 33,816微摩尔/分钟，P = 0.054）。这些结果表明，肠道壁对营养物质的代谢受胃肠道组织葡萄糖可用性的影响，并影响肝脏和外周组织可利用营养物质的模式。
