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通过产肠溶血素鉴定肠出血性大肠杆菌。

Identification of enterohaemorrhagic Escherichia coli by means of their production of enterohaemolysin.

作者信息

Bettelheim K A

机构信息

Biomedical Reference Laboratory, VIDRL, Fairfield Hospital, Victoria, Australia.

出版信息

J Appl Bacteriol. 1995 Aug;79(2):178-80. doi: 10.1111/j.1365-2672.1995.tb00932.x.

DOI:10.1111/j.1365-2672.1995.tb00932.x
PMID:7592113
Abstract

With increasing interest in verocytotoxigenic Escherichia coli (VTEC) being associated with both human and animal infection, a simple system of rapidly identifying the majority of VTEC has been devised. This depends on the fact that most VTEC produce enterohaemolysin, which is rarely produced by non-VTEC. By employing two media, traditional sheep blood agar (SBA) and washed sheep blood agar supplemented with calcium (WSBA-Ca), enterohaemolysin-producing strains can be easily differentiated from other E. coli enabling most VTEC types, including ones belonging to serogroups other than O157, to be isolated.

摘要

随着人们对产志贺毒素大肠杆菌(VTEC)与人类和动物感染之间关系的兴趣日益增加,已设计出一种快速鉴定大多数VTEC的简单系统。这取决于以下事实:大多数VTEC产生肠溶血素,而非VTEC很少产生这种物质。通过使用两种培养基,即传统的绵羊血琼脂(SBA)和补充钙的洗涤绵羊血琼脂(WSBA-Ca),可以轻松地将产生肠溶血素的菌株与其他大肠杆菌区分开来,从而能够分离出大多数VTEC类型,包括属于O157以外血清群的菌株。

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