Miyata M, Aoki T, Inglis V, Yoshida T, Endo M
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan.
J Appl Bacteriol. 1995 Aug;79(2):181-5. doi: 10.1111/j.1365-2672.1995.tb00933.x.
The randomly amplified polymorphic DNA (RAPD) technique was used to analyse the genetic differentiation of 13 strains of Aeromonas salmonicida subsp. salmonicida, and seven strains of Aer. hydrophila. Reproducible profiles of genomic DNA fingerprints were generated by polymerase chain reaction (PCR) using a single randomly designed primer. The RAPD profiles of all the non-motile aeromonads, Aer. salmonicida subsp. salmonicida were identical. However, profiles of the motile aeromonads, Aer. hydrophila differed between isolates. These findings reveal genomic homogeneity in Aer. salmonicida subsp. salmonicida and genetic variety in Aer. hydrophila strains.
采用随机扩增多态性DNA（RAPD）技术分析了13株杀鲑气单胞菌杀鲑亚种和7株嗜水气单胞菌的遗传分化情况。使用单个随机设计的引物，通过聚合酶链反应（PCR）生成了可重复的基因组DNA指纹图谱。所有不运动的气单胞菌，即杀鲑气单胞菌杀鲑亚种的RAPD图谱均相同。然而，运动性气单胞菌嗜水气单胞菌各分离株的图谱存在差异。这些发现揭示了杀鲑气单胞菌杀鲑亚种的基因组同质性和嗜水气单胞菌菌株的遗传多样性。
