Joko S, Numaga J, Fujino Y, Masuda K, Hirata R, Maeda H
Department of Ophthalmology, University of Tokyo School of Medicine.
Nihon Rai Gakkai Zasshi. 1995 Jul;64(2):112-8.
In order to investigate the role of immunogenetic factors in the pathogenesis of uveitis in leprosy, HLA antigens were analyzed between the leprotics with and without uveitis. Sixty-five Japanese leprosy patients comprised 32 with uveitis and 33 without uveitis. Controls consisted of 138 healthy subjects. A lymphocyte cytotoxicity test was used for typing HLA-A, -B, -C, -DR and -DQ antigens. HLA-DR2 genotyping was performed by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and PCR-restriction fragment length polymorphism (RFLP) methods. The frequency of HLA-DR2 antigen was significantly increased in the patients with uveitis as compared with the control subjects (Pc < 0.0005), whereas HLA-DR53 antigen was significantly decreased (Pc < 0.05). At the genomic level the frequency of HLA-DRB11501 was significantly increased in the patients with uveitis and without uveitis as compared with that in the controls. The association with DRB11501 was even stronger in patients with uveitis (odds ratio = 7.1, Pc < 0.000005) than in the patients without uveitis (odds ratio = 4.1, Pc < 0.005). Our results suggest that HLA-DRB1*1501 contribute to the susceptibility to uveitis in the Japanese with leprosy.
为了研究免疫遗传因素在麻风性葡萄膜炎发病机制中的作用，对有葡萄膜炎和无葡萄膜炎的麻风患者进行了HLA抗原分析。65例日本麻风患者中，32例有葡萄膜炎，33例无葡萄膜炎。对照组由138名健康受试者组成。采用淋巴细胞细胞毒性试验对HLA - A、- B、- C、- DR和 - DQ抗原进行分型。通过聚合酶链反应 - 单链构象多态性（PCR - SSCP）和PCR - 限制性片段长度多态性（RFLP）方法进行HLA - DR2基因分型。与对照组相比，葡萄膜炎患者中HLA - DR2抗原频率显著升高（Pc < 0.0005），而HLA - DR53抗原频率显著降低（Pc < 0.05）。在基因水平上，与对照组相比，有葡萄膜炎和无葡萄膜炎的患者中HLA - DRB11501频率均显著升高。葡萄膜炎患者与DRB11501的关联（优势比 = 7.1，Pc < 0.000005）比无葡萄膜炎患者（优势比 = 4.1，Pc < 0.005）更强。我们的结果表明，HLA - DRB1*1501与日本麻风患者发生葡萄膜炎的易感性有关。
