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Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC.大肠杆菌dnaA基因的新等位基因在pSC101的复制中存在缺陷，但在oriC的复制中没有缺陷。
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2
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本文引用的文献

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Evidence of two levels of control of P1 oriR and host oriC replication origins by DNA adenine methylation.DNA腺嘌呤甲基化对P1 oriR和宿主oriC复制起点进行两级控制的证据。
J Bacteriol. 1993 Dec;175(24):7801-7. doi: 10.1128/jb.175.24.7801-7807.1993.
2
Mutations in Escherichia coli dnaA which suppress a dnaX(Ts) polymerization mutation and are dominant when located in the chromosomal allele and recessive on plasmids.大肠杆菌dnaA中的突变，可抑制dnaX（Ts）聚合突变，位于染色体等位基因时呈显性，而在质粒上则呈隐性。
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Reversibility of DnaA protein activity in the 'irreversible' dnaA204 mutant of Escherichia coli.大肠杆菌“不可逆”dnaA204突变体中DnaA蛋白活性的可逆性
Mol Microbiol. 1995 Jan;15(1):141-8. doi: 10.1111/j.1365-2958.1995.tb02228.x.
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Suppression of Escherichia coli dnaA46 mutations by integration of plasmid R100.1. derivatives: constraints imposed by the replication terminus.通过质粒R100.1衍生物的整合抑制大肠杆菌dnaA46突变：复制终点施加的限制
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The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli.dnaN基因编码大肠杆菌DNA聚合酶III全酶的β亚基。
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5391-5. doi: 10.1073/pnas.78.9.5391.
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DNA-protein interaction at the origin of DNA replication of the plasmid pSC101.质粒pSC101 DNA复制起点处的DNA-蛋白质相互作用
Cell. 1983 Dec;35(2 Pt 1):495-502. doi: 10.1016/0092-8674(83)90183-6.
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Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.纯化的DnaA蛋白在大肠杆菌染色体复制起点处启动复制过程中的作用。
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5817-21. doi: 10.1073/pnas.80.19.5817.
8
The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.大肠杆菌K-12的dnaA基因和dnaN基因第一部分的核苷酸序列。
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Enzymatic replication of the origin of the Escherichia coli chromosome.大肠杆菌染色体复制起点的酶促复制
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10
Positive selection for loss of tetracycline resistance.对四环素抗性丧失的正向选择。
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大肠杆菌dnaA基因的新等位基因在pSC101的复制中存在缺陷，但在oriC的复制中没有缺陷。

Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC.

作者信息

Sutton M D, Kaguni J M

机构信息

Department of Biochemistry, Michigan State University, East Lansing 48824-1319, USA.

出版信息

J Bacteriol. 1995 Nov;177(22):6657-65. doi: 10.1128/jb.177.22.6657-6665.1995.

DOI:10.1128/jb.177.22.6657-6665.1995
PMID:7592447
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177522/
Abstract

Five novel alleles of the Escherichia coli dnaA gene that were temperature sensitive in maintenance of pSC101, a plasmid that is dependent on this gene for replication, were isolated. Nucleotide sequence analysis revealed that four of the five alleles arose from single base substitutions, whereas the fifth contained three base substitutions, two of which were silent. Whereas all five alleles were temperature sensitive in vivo for pSC101 maintenance, genetic and biochemical characterization indicated that only two were defective in replication from the chromosomal origin, oriC. As previously characterized mutations are defective in replication for both pSC101 and oriC, the dnaA mutations specifically defective in pSC101 maintenance represent a novel class. We speculate that one or more of these pSC101-specific mutants are defective in interaction with pSC101 RepA protein, which is also required for initiation of plasmid DNA replication.

摘要

分离出了大肠杆菌dnaA基因的五个新等位基因，这些等位基因在维持pSC101（一种依赖该基因进行复制的质粒）时对温度敏感。核苷酸序列分析表明，五个等位基因中的四个来自单碱基替换，而第五个包含三个碱基替换，其中两个是沉默突变。尽管所有五个等位基因在体内对pSC101的维持都对温度敏感，但遗传和生化特征表明，只有两个在从染色体原点oriC进行复制时有缺陷。由于先前鉴定的突变在pSC101和oriC的复制中均有缺陷，因此在pSC101维持中特异性缺陷的dnaA突变代表了一个新类别。我们推测，这些pSC101特异性突变体中的一个或多个在与pSC101 RepA蛋白的相互作用中存在缺陷，而pSC101 RepA蛋白也是质粒DNA复制起始所必需的。