Varani J, Fligiel S E, Inman D R, Beals T F, Hillegas W J
Department of Pathology, University of Michigan, Ann Arbor 48109, USA.
J Biomed Mater Res. 1995 Aug;29(8):993-7. doi: 10.1002/jbm.820290811.
Human squamous epithelial cells produce lower amounts of laminin and fibronectin when cultured on DEAE-dextran than when cultured on gelatin-coated polystyrene (Biotechnol. Bioeng., 33:1235). The epithelial cells also spread much more slowly on DEAE-dextran than they do on gelatin-coated polystyrene. To determine if the low level of matrix production by cells grown on DEAE-dextran contributed to the slowness of cell spreading on this substrate, microcarriers made from DEAE-dextran were treated with exogenous laminin (10 micrograms/cm2 of surface area) and then examined for ability to support cell adhesion. Squamous epithelial cells spread as rapidly on the laminin-treated DEAE-dextran as they did on gelatin-coated polystyrene (much more rapidly than on untreated DEAE-dextran). This indicates 1) that laminin can bind to DEAE-dextran in a fashion that is biologically usable by anchorage-dependent cells, and 2) that when laminin is bound to DEAE-dextran, the failure of squamous epithelial cells to rapidly spread is overcome. These data support the hypothesis that failure of the cells to synthesize an intact extracellular matrix on DEAE-dextran is responsible, at least in part, for the slowness with which cells spread on this substrate.
人鳞状上皮细胞在DEAE - 葡聚糖上培养时产生的层粘连蛋白和纤连蛋白量比在明胶包被的聚苯乙烯上培养时少（《生物技术与生物工程》，33:1235）。上皮细胞在DEAE - 葡聚糖上的铺展也比在明胶包被的聚苯乙烯上慢得多。为了确定在DEAE - 葡聚糖上生长的细胞产生的低水平基质是否导致了细胞在该基质上铺展缓慢，用外源性层粘连蛋白（10微克/平方厘米表面积）处理由DEAE - 葡聚糖制成的微载体，然后检测其支持细胞黏附的能力。鳞状上皮细胞在经层粘连蛋白处理的DEAE - 葡聚糖上的铺展速度与在明胶包被的聚苯乙烯上一样快（比在未处理的DEAE - 葡聚糖上快得多）。这表明1）层粘连蛋白可以以一种依赖锚定的细胞能够生物学利用的方式与DEAE - 葡聚糖结合，以及2）当层粘连蛋白与DEAE - 葡聚糖结合时，鳞状上皮细胞不能快速铺展的问题得以克服。这些数据支持了这样的假设，即细胞在DEAE - 葡聚糖上不能合成完整的细胞外基质至少部分地导致了细胞在该基质上铺展缓慢。
