Shimaoka S, Tsuboi R, Jindo T, Imai R, Takamori K, Rubin J S, Ogawa H
Department of Dermatology, Juntendo University, School of Medicine, Tokyo, Japan.
J Cell Physiol. 1995 Nov;165(2):333-8. doi: 10.1002/jcp.1041650214.
Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional polypeptide which acts as mitogen, motogen, or morphogen. In this study, we examined the effect of HGF/SF on human hair growth using organ and cell culture systems. HGF/SF was found to stimulate hair length and DNA synthesis in hair follicles at increasing concentrations up to 10 ng/ml (P < 0.05 and P < 0.01, respectively). HGF/SF stimulated [3H]thymidine incorporation by hair bulb-derived keratinocytes with the strongest response at 30 ng/ml of HGF/SF (P < 0.05). Cultured follicular papilla cells secreted HGF/SF, measured by an enzyme-linked immunoassay, in response to interleukin 1-alpha (IL1-alpha, 10 ng/ml), tumor necrosis factor-alpha (TNF-alpha, 10 ng/ml), or tetradecanoylphorbolacetate (100 nM) at levels ranging from 0.2 to 0.3 ng/mg protein/48 h. HGF/SF mRNA expressions, measured by the reverse transcription-polymerase chain reaction, were detected in follicular papilla cells, and were also stimulated by the three reagents. Transforming growth factor-beta (10 ng/ml) suppressed both protein and mRNA levels. These results suggest that hair follicle elongation induced by HGF/SF in organ culture occurs partly due to the mitogenic activity of HGF/SF expressed in follicular papilla cells on hair bulb-derived keratinocytes.
肝细胞生长因子/分散因子（HGF/SF）是一种多功能多肽，具有促有丝分裂原、促运动因子或形态发生素的作用。在本研究中，我们使用器官和细胞培养系统研究了HGF/SF对人类毛发生长的影响。发现HGF/SF在浓度增加至10 ng/ml时可刺激毛囊的毛发长度和DNA合成（分别为P < 0.05和P < 0.01）。HGF/SF刺激毛球来源的角质形成细胞掺入[3H]胸苷，在HGF/SF浓度为30 ng/ml时反应最强（P < 0.05）。通过酶联免疫测定法测量，培养的毛囊乳头细胞在受到白细胞介素1-α（IL1-α，10 ng/ml）、肿瘤坏死因子-α（TNF-α，10 ng/ml）或十四酰佛波醇乙酸酯（100 nM）刺激时分泌HGF/SF，分泌水平为0.2至0.3 ng/mg蛋白质/48小时。通过逆转录-聚合酶链反应测量，在毛囊乳头细胞中检测到HGF/SF mRNA表达，并且这三种试剂也可刺激其表达。转化生长因子-β（10 ng/ml）可抑制蛋白质和mRNA水平。这些结果表明，在器官培养中HGF/SF诱导的毛囊伸长部分是由于毛囊乳头细胞中表达的HGF/SF对毛球来源的角质形成细胞的促有丝分裂活性所致。
