Arai T, Kusubata M, Kohsaka T, Shiraiwa M, Sugawara K, Takahara H
Laboratory of Biochemistry, School of Agriculture, Ibaraki University, Japan.
J Cell Biochem. 1995 Jul;58(3):269-78. doi: 10.1002/jcb.240580302.
Peptidylarginine deiminase is localized in the cytosol of the luminal and glandular epithelia of the nonpregnant murine uterus and its expression is regulated by sex hormones [Takahara et al., [1989]: J Biol Chem 264, 13361-13368; Takahara et al. [1992]: J Biol Chem 267,520-525]. Here, we demonstrate that changes occur in the enzyme level in the mouse uterus during pregnancy and parturition. After a rapid decrease in enzymatic activity from day 1 to day 5 of pregnancy, the activity sharply increased during the middle stage of pregnancy (day 8 to day 10) and then gradually decreased during late pregnancy. Expression of the enzyme occurred only in the decidual cells that had differentiated from endometrial stroma cells surrounding the implantation site. The immunochemical properties of the enzyme expressed in the decidualized cells was indistinguishable from those in the uterine epithelia. These results suggest that peptidylarginine deiminase has important roles in decidual cells and not just in the epithelia of the nonpregnant uterus. Moreover, the level of enzyme activity increased slightly just before parturition (day 17), and then decreased during the 12 h period after parturition. The tissue localization of the enzyme expressed around the time of parturition changed from decidua to the luminal and glandular epithelia. Semiquantitative analyses of the enzyme mRNA content in the pregnant uteri showed a remarkable increase from day 7 leading to the onset of the enzyme synthesis in the decidual cells. After reaching the maximal level at day 12, small peaks in the mRNA level were observed at two times during late pregnancy. Since these serial changes in the mRNA level did not correlate with changes in sex hormones, the expression of decidual peptidylarginine deiminase seemed to be controlled by factors other than sex hormones.
肽基精氨酸脱亚氨酶定位于未孕小鼠子宫腔上皮和腺上皮的细胞质中，其表达受性激素调节[高桥等人，[1989]：《生物化学杂志》264，13361 - 13368；高桥等人[1992]：《生物化学杂志》267，520 - 525]。在此，我们证明在小鼠子宫妊娠和分娩期间酶水平会发生变化。妊娠第1天到第5天酶活性迅速下降，在妊娠中期（第8天到第10天）活性急剧增加，然后在妊娠后期逐渐下降。该酶仅在与着床部位周围子宫内膜基质细胞分化而来的蜕膜细胞中表达。在蜕膜化细胞中表达的该酶的免疫化学特性与子宫上皮中的无异。这些结果表明肽基精氨酸脱亚氨酶在蜕膜细胞中具有重要作用，而不仅仅在未孕子宫的上皮中。此外，在分娩前（第17天）酶活性水平略有升高，然后在分娩后的12小时内下降。分娩时表达的该酶的组织定位从蜕膜转变为腔上皮和腺上皮。对妊娠子宫中酶mRNA含量的半定量分析显示，从第7天开始有显著增加，导致蜕膜细胞中酶合成开始。在第12天达到最高水平后，在妊娠后期观察到mRNA水平有两次小峰值。由于mRNA水平的这些系列变化与性激素变化无关，蜕膜肽基精氨酸脱亚氨酶的表达似乎受性激素以外的因素控制。
