Zhao M L, Fritz R B
Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226, USA.
J Neuroimmunol. 1995 Sep;61(2):179-84. doi: 10.1016/0165-5728(95)00090-o.
Experimental autoimmune encephalomyelitis was induced in SJL/J mice by adoptive transfer of a MBP exon-2 peptide-specific T cell line. The T cell line, when tested for antigen specificity, reacted strongly with exon-2 peptide, but not with MBP peptides pAc1-11, p43-88, p89-101 or PLP p139-151. The specificity of splenic or lymph node T cells isolated from mice with acute or first relapse EAE induced by adoptive transfer of the exon-2-specific T cell line was identical to the transferred line. Splenocytes or lymphocytes isolated from mice at the second relapse were reactive with MBP p43-88, p89-101 and PLP p139-151 in addition to exon-2 peptide and MBP peptide Ac1-11. T cell lines selected by culture with MBP exon-2 peptide or PLP p139-151 from splenic cells from mice with relapsing EAE were weakly encephalitogenic; however, T cell lines selected from the same mice with MBP pAc1-11 were not encephalitogenic. T cells from the exon-2 and p139-151 T cell lines primed recipients for rapid onset severe EAE, whereas the pAc1-11 T cell line did not. T cells from the exon-2-specific line did not express V beta 17a+ TCR; however, peptide-specific T cell lines derived from the spleens of relapsing animals did express this TCR gene segment providing direct evidence of recruitment and sensitization of recipient T cells.
通过过继转移髓鞘碱性蛋白（MBP）外显子2肽特异性T细胞系，在SJL/J小鼠中诱导实验性自身免疫性脑脊髓炎（EAE）。该T细胞系在检测抗原特异性时，与外显子2肽强烈反应，但与MBP肽pAc1 - 11、p43 - 88、p89 - 101或髓磷脂蛋白脂蛋白（PLP）p139 - 151无反应。从通过过继转移外显子2特异性T细胞系诱导的急性或首次复发EAE小鼠中分离的脾或淋巴结T细胞的特异性与转移的细胞系相同。从第二次复发小鼠中分离的脾细胞或淋巴细胞除了与外显子2肽和MBP肽Ac1 - 11反应外，还与MBP p43 - 88、p89 - 101和PLP p139 - 151反应。用复发型EAE小鼠脾细胞中的MBP外显子2肽或PLP p139 - 151培养选择的T细胞系致脑炎作用较弱；然而，用MBP pAc1 - 11从相同小鼠中选择的T细胞系无致脑炎作用。来自外显子2和p139 - 151 T细胞系的T细胞使受体引发快速发作的严重EAE，而pAc1 - 11 T细胞系则不然。来自外显子2特异性细胞系的T细胞不表达Vβ17a + TCR；然而，来自复发动物脾脏的肽特异性T细胞系确实表达了该TCR基因片段，这为受体T细胞的募集和致敏提供了直接证据。
