Kruszewska B, Felten S Y, Moynihan J A
Center for Psychoneuroimmunology Research, University of Rochester Medical Center, NY 14642, USA.
J Immunol. 1995 Nov 15;155(10):4613-20.
It is becoming clear that immune responses are subject to modulation by the sympathetic nervous system. We examined the effect of chemical sympathectomy (to ablate peripheral sympathetic nerve fibers) on cytokine and Ab production in two strains of mice that are known to differ in their response to a variety of pathogens and in the dominant types of cytokines produced. C57Bl/6J mice produce a strong cell-mediated response, characterized by production of IL-2 and IFN-gamma, whereas BALB/cJ have a dominant humoral response, with production of IL-4 and IL-10. Animals were denervated by injection with 6-hydroxydopamine and immunized with keyhole limpet hemocyanin, and spleens were removed at various times after immunization. Denervation significantly increased the keyhole-limpet-hemocyanin-stimulated in vitro proliferation and IL-2 and IL-4 production by splenocytes from both strains. The increases were prevented by pretreatment with desipramine, which blocks the uptake of 6-hydroxydopamine into the nerve fibers and subsequent nerve fiber destruction. Serum titers of IgM, IgG, IgG1, and IgG2a were also enhanced in the C57Bl/6J strain; BALB/cJ mice had a small increase in IgG1 only. These results suggest that one function of splenic innervation and transmitter release may be to modulate T helper cytokines, thereby partially regulating immune effector function. Our evidence is consistent with a model of immune regulation in which removal of sympathetic nervous system input enhances at least some parameters of immune responses.
越来越明显的是，免疫反应会受到交感神经系统的调节。我们研究了化学性交感神经切除术（切除外周交感神经纤维）对两种小鼠细胞因子和抗体产生的影响，已知这两种小鼠对多种病原体的反应以及产生的主要细胞因子类型存在差异。C57Bl/6J小鼠产生强烈的细胞介导反应，其特征是产生IL-2和IFN-γ，而BALB/cJ小鼠则以产生IL-4和IL-10为主的体液反应占主导。通过注射6-羟基多巴胺使动物去神经支配，并用钥孔血蓝蛋白免疫，在免疫后的不同时间取出脾脏。去神经支配显著增加了两种品系脾细胞对钥孔血蓝蛋白刺激的体外增殖以及IL-2和IL-4的产生。预先用去甲丙咪嗪处理可阻止这种增加，去甲丙咪嗪可阻断6-羟基多巴胺进入神经纤维并随后破坏神经纤维。C57Bl/6J品系的IgM、IgG、IgG1和IgG2a血清滴度也有所提高；BALB/cJ小鼠仅IgG1有小幅增加。这些结果表明，脾神经支配和递质释放的一个功能可能是调节辅助性T细胞细胞因子，从而部分调节免疫效应功能。我们的证据与一种免疫调节模型一致，即在该模型中，去除交感神经系统输入会增强免疫反应的至少一些参数。
