Möller L V, Regelink A G, Grasselier H, Dankert-Roelse J E, Dankert J, van Alphen L
Department of Medical Microbiology, University of Amsterdam, Netherlands.
J Infect Dis. 1995 Nov;172(5):1388-92. doi: 10.1093/infdis/172.5.1388.
To investigate the epidemiology of nontypeable Haemophilus influenzae in the respiratory tract of cystic fibrosis (CF) patients, H. influenzae isolates from sputum specimens of 40 CF patients were analyzed longitudinally for 2 years. The isolates were characterized by analysis of the major outer membrane protein (MOMP) patterns. MOMP variant H. influenzae strains were discriminated from distinct strains by randomly amplified polymorphic DNA analysis of genomic DNA. Multiple H. influenzae strains and MOMP variant strains were isolated from single sputum specimens of 29 patients. In 22 patients, a distinct H. influenzae strain persisted over time (median persistence, 8 months; range 2-24). In general, the appearance of MOMP variant strains did not coincide with the occurrence of exacerbations.
为研究非分型流感嗜血杆菌在囊性纤维化（CF）患者呼吸道中的流行病学情况，对40例CF患者痰液标本中的流感嗜血杆菌分离株进行了为期2年的纵向分析。通过分析主要外膜蛋白（MOMP）模式对分离株进行鉴定。通过对基因组DNA进行随机扩增多态性DNA分析，将MOMP变异型流感嗜血杆菌菌株与不同菌株区分开来。从29例患者的单个痰液标本中分离出多种流感嗜血杆菌菌株和MOMP变异菌株。在22例患者中，一种独特的流感嗜血杆菌菌株长期存在（中位持续时间为8个月；范围为2 - 24个月）。一般来说，MOMP变异菌株的出现与病情加重的发生并不一致。
