Xu G, Fujita J, Negayama K, Ohnishi T, Miyawaki H, Hojo S, Takigawa K, Okada H, Yamaji Y, Takahara J
Kansenshogaku Zasshi. 1995 Aug;69(8):864-72. doi: 10.11150/kansenshogakuzasshi1970.69.864.
Recently, it has been suggested that macrolide antibiotics act as immunomodulators. In this study, we evaluated the effect of clarithromycin (CAM) on macrophage function. We used the mouse macrophage cell line, J774.1. The following direct effects of CAM on macrophage function were evaluated: chemotaxis to CAM, chemokinetic effect of CAM, and the effect of CAM on macrophage growth. In order to examine the indirect effects of CAM on macrophage functions, we preincubated macrophages with several concentrations of CAM and then removed the CAM. Thereafter, the phagocytosis of beads, cytocidal activity against Candida albicans, and chemotaxis to lipopolysaccharide were evaluated. In addition, the indirect effects of CAM on endoxan (4 mg/ml) treated macrophage phagocytosis, cytocidal activity, and chemotaxis were evaluated. CAM (at the concentration between 0.04 and 0.2 microgram/ml) directly stimulated macrophage chemotaxis and chemokinesis. In addition, CAM dose-dependently stimulated the growth of macrophages. CAM pretreatment (for 4 hours at the concentrations between 0.04 and 0.2 microgram/ml) stimulated macrophage phagocytosis, cytocidal activity against Candida albicans, and chemotaxis to lipopolysaccharide. In addition, CAM recovered macrophage phagocytosis, cytocidal activity, and chemotaxis which were decreased after endoxan exposure. These results suggest that CAM has direct and indirect effects on macrophage functions.
最近，有人提出大环内酯类抗生素可作为免疫调节剂。在本研究中，我们评估了克拉霉素（CAM）对巨噬细胞功能的影响。我们使用了小鼠巨噬细胞系J774.1。评估了CAM对巨噬细胞功能的以下直接作用：对CAM的趋化性、CAM的化学动力学效应以及CAM对巨噬细胞生长的影响。为了研究CAM对巨噬细胞功能的间接作用，我们用几种浓度的CAM对巨噬细胞进行预孵育，然后去除CAM。此后，评估珠子的吞噬作用、对白色念珠菌的杀细胞活性以及对脂多糖的趋化性。此外，还评估了CAM对内毒素（4 mg/ml）处理的巨噬细胞吞噬作用、杀细胞活性和趋化性的间接影响。CAM（浓度在0.04至0.2微克/毫升之间）直接刺激巨噬细胞趋化性和化学运动性。此外，CAM剂量依赖性地刺激巨噬细胞生长。CAM预处理（浓度在0.04至0.2微克/毫升之间，处理4小时）刺激巨噬细胞吞噬作用、对白色念珠菌的杀细胞活性以及对脂多糖的趋化性。此外，CAM恢复了内毒素暴露后降低的巨噬细胞吞噬作用、杀细胞活性和趋化性。这些结果表明CAM对巨噬细胞功能有直接和间接影响。
