Li Z, Wang X, Guo Y, Zhao J
Bacteriological Laboratory, Second Affiliated Hospital of Hebie Medical College.
Kansenshogaku Zasshi. 1995 Aug;69(8):924-7. doi: 10.11150/kansenshogakuzasshi1970.69.924.
Fifty clinical isolates of Pseudomonas aeruginosa were tested for inhibition of growth of clinical isolates of Escherichia coli, Salmonella infantis, Klebsiella pneumoniae and other Gram-negative bacteria in the authors' laboratory. Pseudomonas aeruginosa was strongly active against both E. coli and Enterobacter cloacae, with 89.4% and 94.7% inhibition respectively, but weakly active against S. infantis, K. pneumoniae and Proteus mirabilis with 56.3%, 48.8% and 23.8% inhibition, respectively. The pigmented strains were found to have stronger antimicrobial activity than the unpigmented strains. Pyocyanin, the major metabolite of Pseudomonas aeruginosa, has been shown to inhibit Escherichia coli, Proteus spp. and other Gram-negative bacteria, by research with a few strains of P. aeruginosa and a single inhibited strain. However, little attempt has been made to determine the inhibitory action of many strains of P. aeruginosa against a large number of clinical isolates such as Escherichia spp., Klebsiella spp., and Salmonella spp., up to now. For this reason, in this study we examined 50 randomly selected clinical isolates of P. aeruginosa for inhibition of growth of a wide range of Gram-negative bacteria, including 30 strains of E. coli, 30 of K. pneumoniae, 30 of S. infantis, 6 of Enterobacter cloacae and 9 of Proteus mirabilis.
在作者的实验室中，对50株铜绿假单胞菌临床分离株抑制大肠杆菌、婴儿沙门氏菌、肺炎克雷伯菌和其他革兰氏阴性菌临床分离株生长的情况进行了测试。铜绿假单胞菌对大肠杆菌和阴沟肠杆菌均有较强活性，抑制率分别为89.4%和94.7%，但对婴儿沙门氏菌、肺炎克雷伯菌和奇异变形杆菌活性较弱，抑制率分别为56.3%、48.8%和23.8%。发现有色素的菌株比无色素的菌株具有更强的抗菌活性。通过对几株铜绿假单胞菌和单一被抑制菌株的研究表明，铜绿假单胞菌的主要代谢产物绿脓菌素可抑制大肠杆菌、变形杆菌属和其他革兰氏阴性菌。然而，到目前为止，几乎没有人尝试确定许多铜绿假单胞菌菌株对大量临床分离株（如埃希氏菌属、克雷伯菌属和沙门氏菌属）的抑制作用。因此，在本研究中，我们随机选取了50株铜绿假单胞菌临床分离株，检测其对多种革兰氏阴性菌生长的抑制情况，这些革兰氏阴性菌包括30株大肠杆菌、30株肺炎克雷伯菌、30株婴儿沙门氏菌、6株阴沟肠杆菌和9株奇异变形杆菌。
