Calabrese B, Pellegrino M
Dipartimento di Fisiologia e Biochimica, Università di Pisa, Italy.
J Exp Biol. 1995 Sep;198(Pt 9):1989-94. doi: 10.1242/jeb.198.9.1989.
The regeneration pattern of two identified central neurones was studied in the leech Hirudo medicinalis. Anterior pagoda (AP) and mechanosensory touch-sensitive (T) neurones were stained in adult segmental ganglia, maintained in culture for 6-10 days. AP neurones, which normally project only to the contralateral nerve roots, sprouted extensively in all the available nerve paths during regeneration. Mechanosensory T cells, in the same experimental conditions, showed only a moderate growth and did not change their normal pattern of axonal projections. The observed differences in the growth pattern might account for the different electrophysiological responses to axotomy exhibited by the two types of neurone. Interruption of interganglionic connectives induced a moderate and stereotyped remodelling of the morphology of intact AP neurones, which was reminiscent of that transiently exhibited during embryonic development. This response was observed in 25% of the AP neurones we examined.
在医用水蛭中研究了两种已确定的中枢神经元的再生模式。在前体节神经节中对前塔（AP）神经元和机械感觉触觉敏感（T）神经元进行染色，并在培养中维持6 - 10天。AP神经元通常仅投射到对侧神经根，在再生过程中在所有可用神经路径中广泛发芽。在相同实验条件下，机械感觉T细胞仅显示适度生长，并且其轴突投射的正常模式没有改变。观察到的生长模式差异可能解释了两种神经元对轴突切断所表现出的不同电生理反应。神经节间连接的中断诱导了完整AP神经元形态的适度且定型的重塑，这让人想起胚胎发育期间短暂表现出的重塑。在我们检查的25%的AP神经元中观察到了这种反应。
