Saku K, Liu R, Jimi S, Matsuo K, Yamamoto K, Yanagita T, Arakawa K
Department of Internal Medicine, Fukuoka University School of Medicine,Japan.
Jpn Circ J. 1995 May;59(5):292-8. doi: 10.1253/jcj.59.292.
The combined effects of pravastatin and probucol on high- density lipoprotein (HDL) apolipoprotein (apo) A-I kinetics in cholesterol (Ch)-fed rabbits were investigated. Japanese White rabbits were treated with 0.15% pravastatin and 0.5% Ch (group 1) or 0.15% pravastatin plus 1% probucol and 0.5% Ch (group 2) for 2 months. After treatment, the serum total cholesterol levels in groups 1 and 2 had significantly (p < 0.01) increased (37.4 +/- 6.7 mg/dl vs 117.1 +/- 46.4 mg/dl, and 31.4 +/- 4.9 mg/dl vs 143.0 +/- 84.5 mg /dl, respectively). The serum HDL-cholesterol levels in both groups decreased (18.2 +/- 2.8 mg/dl, vs 16.2 +/- 3.7 mg/dl p < 0.01 for group 1; 18.2 +/- 1.9 mg/dl vs 15.5 +/- 4.5 mg/dl, ns for group 2). Apo A-I kinetics were assessed by injecting (125)I-labeled HDL intravenously into both groups of rabbits, and taking blood samples periodically for 6 days. Kinetic parameters calculated from apo A-I specific radioactivity decay curves showed that the apo A-I fractional catabolic rates in rabbits fed pravastatin and Ch (group 1) were significantly less than those in rabbits fed pravastatin plus probucol and Ch (group 2) (0.546 +/- 0.017 /day vs 0.730 +/- 0.126 /day, p < 0.05), while the synthetic rate of apo A-I was lower in group 2 than in group 1 (14.76 +/- 1.71 mg/kg per day vs 11.21 +/- 2.38 mg/kg per day, respectively, p < 0.01). These data indicate that pravastatin and probucol have different effects on HDL-apo A-I kinetics in a diet which includes cholesterol.
研究了普伐他汀和普罗布考联合使用对喂食胆固醇（Ch）的家兔高密度脂蛋白（HDL）载脂蛋白（apo）A-I动力学的影响。将日本白兔分为两组，一组用0.15%普伐他汀和0.5% Ch处理（第1组），另一组用0.15%普伐他汀加1%普罗布考和0.5% Ch处理（第2组），持续2个月。处理后，第1组和第2组的血清总胆固醇水平均显著升高（p < 0.01）（分别为37.4±6.7mg/dl 对117.1±46.4mg/dl，以及31.4±4.9mg/dl对143.0±84.5mg/dl）。两组的血清HDL胆固醇水平均下降（第1组：18.2±2.8mg/dl对16.2±3.7mg/dl，p < 0.01；第2组：18.2±1.9mg/dl对15.5±4.5mg/dl，无显著差异）。通过向两组家兔静脉注射（125）I标记的HDL，并在6天内定期采集血样来评估apo A-I动力学。根据apo A-I比放射性衰变曲线计算的动力学参数表明，喂食普伐他汀和Ch的家兔（第1组）的apo A-I分解代谢率显著低于喂食普伐他汀加普罗布考和Ch的家兔（第2组）（0.546±0.017/天对0.730±0.126/天，p < 0.05），而第2组的apo A-I合成率低于第1组（分别为14.76±1.71mg/kg per day对11.21±2.38mg/kg per day，p < 0.01）。这些数据表明，在含有胆固醇的饮食中，普伐他汀和普罗布考对HDL-apo A-I动力学有不同影响。
