水合桑色素对人低密度脂蛋白的抗氧化作用。

Antioxidation of human low density lipoprotein by morin hydrate.

作者信息

Wu T W, Fung K P, Yang C C, Weisel R D

机构信息

Department of Clinical Biochemistry, University of Toronto, Ontario, Canada.

出版信息

Life Sci. 1995;57(3):PL51-6. doi: 10.1016/0024-3205(95)00274-a.

DOI:10.1016/0024-3205(95)00274-a

PMID:7596225

Abstract

Oxidative modification of low density lipoprotein (LDL) has been suggested to be a risk factor for the development of atherosclerosis. Agents which can protect LDL from oxidation may be useful in preventing atherogenesis. Here, we found that morin hydrate, at 100 microM concentration, effectively inhibits Cu(2+)-induced oxidation of LDL. The oxidation of LDL was assessed by agarose gel electrophoresis. This was further studied by measuring the increased values of the malondialdehyde equivalents and the decreased numbers of reactive amino groups on oxidized LDL. Trolox, at equimolar concentrations, exhibit similar effects in preventing oxidation of LDL.

摘要

低密度脂蛋白（LDL）的氧化修饰被认为是动脉粥样硬化发生发展的一个危险因素。能够保护LDL不被氧化的药物可能有助于预防动脉粥样硬化的形成。在此，我们发现，浓度为100微摩尔的水合桑色素能有效抑制铜离子（Cu(2+)）诱导的LDL氧化。LDL的氧化通过琼脂糖凝胶电泳进行评估。通过测量氧化型LDL上丙二醛当量的增加值和反应性氨基数量的减少来进一步研究。等摩尔浓度的曲洛昔芬在预防LDL氧化方面表现出类似的效果。