T-T嘧啶（6-4）嘧啶酮紫外线光产物在酵母中的致突变性比在大肠杆菌中低得多。
The T-T pyrimidine (6-4) pyrimidinone UV photoproduct is much less mutagenic in yeast than in Escherichia coli.
作者信息
Gibbs P E, Borden A, Lawrence C W
机构信息
Department of Biophysics, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
出版信息
Nucleic Acids Res. 1995 Jun 11;23(11):1919-22. doi: 10.1093/nar/23.11.1919.
Abstract
We have examined the mutagenic properties of the T-T pyrimidine (6-4) pyrimidinone UV photoproduct in Saccharomyces cerevisiae, transforming the yeast cells either with single-stranded vectors that carried this adduct at a unique site or with gapped duplex vectors in which the adduct was located within a 28 nt single-stranded region. In an earlier study with SOS-induced Escherichia coli, we found that this photoproduct is highly mutagenic, specifically generating 3' T-->C substitutions in >85% of replicated molecules, and ascribed this specificity to the formation of a stable guanine-pyrimidinone mispair via hydrogen bonds at N-3 and O-2. In contrast, this adduct is very much less mutagenic in yeast, with 60-70% of molecules being replicated accurately and only 12-20% of them exhibiting 3' T-->C substitutions. The enhanced accuracy may reflect the ability of a yeast DNA polymerase, but not E.coli DNA polymerase III, to trap the adduct in a configuration favorable for the formation of an adenine-pyrimidinone base pair.
摘要
我们检测了酿酒酵母中T-T嘧啶（6-4）嘧啶酮紫外线光产物的诱变特性，通过用在独特位点携带该加合物的单链载体或加合物位于28个核苷酸单链区域内的缺口双链载体转化酵母细胞。在早期对SOS诱导的大肠杆菌的研究中，我们发现这种光产物具有高度诱变作用，在>85%的复制分子中特异性产生3'端T→C替换，并将这种特异性归因于通过N-3和O-2处的氢键形成稳定的鸟嘌呤-嘧啶酮错配。相比之下，这种加合物在酵母中的诱变作用要小得多，60-70%的分子能准确复制，只有12-20%的分子表现出3'端T→C替换。准确性的提高可能反映了酵母DNA聚合酶而非大肠杆菌DNA聚合酶III将加合物捕获在有利于形成腺嘌呤-嘧啶酮碱基对的构型中的能力。
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