草履虫IESs的共有反向末端重复序列：与Tc1相关转座子和游仆虫Tec转座子末端的相似性
Consensus inverted terminal repeat sequence of Paramecium IESs: resemblance to termini of Tc1-related and Euplotes Tec transposons.
作者信息
Klobutcher L A, Herrick G
机构信息
Department of Biochemistry, University of Connecticut Health Center, Farmington 06030, USA.
出版信息
Nucleic Acids Res. 1995 Jun 11;23(11):2006-13. doi: 10.1093/nar/23.11.2006.
Abstract
During the formation of a transcriptionally active macronucleus, ciliated protozoa excise large numbers of interstitial segments of DNA (internal eliminated sequences; IESs) from their chromosomes. In this study we analyze the published sequences of 20 IESs that interrupt surface protein genes of Paramecium and identify a consensus inverted terminal repeat. This sequence is similar to the ends of the Tc1-related transposons found in nematodes and other metazoans, as well as to both the ends of the Tec transposons and at least some of the IESs in the distantly related ciliate Euplotes crassus. The results of these analyses bolster previous proposals that IESs were created by transposition.
摘要
在转录活性大核形成过程中，纤毛原生动物从其染色体上切除大量的DNA间隔片段（内部消除序列；IESs）。在本研究中，我们分析了20个中断草履虫表面蛋白基因的IESs的已发表序列，并鉴定出一个共有反向末端重复序列。该序列类似于线虫和其他后生动物中发现的Tc1相关转座子的末端，也类似于Tec转座子的两端以及远缘纤毛虫粗尾真核生物中至少一些IESs的末端。这些分析结果支持了先前关于IESs是通过转座产生的提议。
相似文献
1
Consensus inverted terminal repeat sequence of Paramecium IESs: resemblance to termini of Tc1-related and Euplotes Tec transposons.草履虫IESs的共有反向末端重复序列：与Tc1相关转座子和游仆虫Tec转座子末端的相似性
Nucleic Acids Res. 1995 Jun 11;23(11):2006-13. doi: 10.1093/nar/23.11.2006.
2
Internal eliminated sequences interrupting the Oxytricha 81 locus: allelic divergence, conservation, conversions, and possible transposon origins.中断嗜热四膜虫81位点的内部消除序列：等位基因分歧、保守性、转换及可能的转座子起源
Mol Biol Evol. 1996 Dec;13(10):1351-62. doi: 10.1093/oxfordjournals.molbev.a025581.
3
Identification of single nucleotide mutations that prevent developmentally programmed DNA elimination in Paramecium tetraurelia.鉴定阻止四膜虫发育程序性DNA消除的单核苷酸突变
J Eukaryot Microbiol. 2004 Nov-Dec;51(6):664-9. doi: 10.1111/j.1550-7408.2004.tb00606.x.
4
Tec3, a new developmentally eliminated DNA element in Euplotes crassus.Tec3，一种在粗壮真核游仆虫中全新的发育过程中被消除的DNA元件。
Eukaryot Cell. 2003 Feb;2(1):103-14. doi: 10.1128/EC.2.1.103-114.2003.
5
A mutation in Paramecium tetraurelia reveals functional and structural features of developmentally excised DNA elements.四膜虫的一种突变揭示了发育性切除的DNA元件的功能和结构特征。
Genetics. 1998 Jan;148(1):139-49. doi: 10.1093/genetics/148.1.139.
6
The Paramecium germline genome provides a niche for intragenic parasitic DNA: evolutionary dynamics of internal eliminated sequences.草履虫生殖系基因组为基因内寄生 DNA 提供了一个小生境：内部缺失序列的进化动态。
PLoS Genet. 2012;8(10):e1002984. doi: 10.1371/journal.pgen.1002984. Epub 2012 Oct 4.
7
Preferential cleavage of Paramecium DNA mediated by the C. elegans Tc1 transposase in vitro.秀丽隐杆线虫Tc1转座酶体外介导的草履虫DNA优先切割
Genes Genet Syst. 2003 Dec;78(6):391-8. doi: 10.1266/ggs.78.391.
8
Developmentally excised DNA sequences in Euplotes crassus capable of forming G quartets.在粗壮真核浮游生物中能够形成G四联体的发育切除DNA序列。
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):1979-83. doi: 10.1073/pnas.92.6.1979.
9
A repetitive DNA sequence in Paramecium macronuclei is related to the beta subunit of G proteins.草履虫大核中的一个重复DNA序列与G蛋白的β亚基相关。
Nucleic Acids Res. 1992 Oct 25;20(20):5397-402. doi: 10.1093/nar/20.20.5397.
10
High fidelity developmental excision of Tec1 transposons and internal eliminated sequences in Euplotes crassus.在粗壮真核游仆虫中Tec1转座子和内部消除序列的高保真发育切除
Nucleic Acids Res. 1991 Jun 25;19(12):3229-36. doi: 10.1093/nar/19.12.3229.
引用本文的文献
1
Fidelity, specialization, and evolution of Paramecium PolX DNA polymerases involved in programmed double-strand break DNA repair.参与程序性双链断裂DNA修复的草履虫PolX DNA聚合酶的保真度、特异性及进化
Nucleic Acids Res. 2025 Aug 11;53(15). doi: 10.1093/nar/gkaf786.
2
ISWI1 complex proteins facilitate developmental genome editing in .ISWI1复合蛋白促进了……中的发育基因组编辑。 （原文中“in”后面缺少具体内容）
Genome Res. 2025 Jan 22;35(1):93-108. doi: 10.1101/gr.278402.123.
3
Two paralogous PHD finger proteins participate in natural genome editing in Paramecium tetraurelia.两个旁系同源的 PHD 手指蛋白参与了 Paramecium tetraurelia 的自然基因组编辑。
J Cell Sci. 2024 Aug 15;137(16). doi: 10.1242/jcs.261979. Epub 2024 Aug 30.
4
From germline genome to highly fragmented somatic genome: genome-wide DNA rearrangement during the sexual process in ciliated protists.从种系基因组到高度碎片化的体细胞基因组：纤毛虫有性生殖过程中的全基因组DNA重排
Mar Life Sci Technol. 2024 Feb 12;6(1):31-49. doi: 10.1007/s42995-023-00213-x. eCollection 2024 Feb.
5
Application of RNA interference and protein localization to investigate housekeeping and developmentally regulated genes in the emerging model protozoan Paramecium caudatum.应用RNA干扰和蛋白质定位技术研究新出现的模式原生动物尾草履虫中的管家基因和发育调控基因。
Commun Biol. 2024 Feb 19;7(1):204. doi: 10.1038/s42003-024-05906-2.
6
Programmed chromosome fragmentation in ciliated protozoa: multiple means to chromosome ends.纤毛原生动物中的程序性染色体碎裂：到达染色体末端的多种方式。
Microbiol Mol Biol Rev. 2023 Dec 20;87(4):e0018422. doi: 10.1128/mmbr.00184-22. Epub 2023 Nov 27.
7
Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle.代际核串扰将基因表达的控制与草履虫有性周期中的程序性基因组重排联系起来。
Nucleic Acids Res. 2023 Dec 11;51(22):12337-12351. doi: 10.1093/nar/gkad1006.
8
MITE infestation accommodated by genome editing in the germline genome of the ciliate .纤毛虫种系基因组中的基因编辑可容纳螨类侵染
Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2213985120. doi: 10.1073/pnas.2213985120. Epub 2023 Jan 20.
9
Comparative genomics reveals insight into the evolutionary origin of massively scrambled genomes.比较基因组学揭示了大规模基因组混乱进化起源的见解。
Elife. 2022 Nov 24;11:e82979. doi: 10.7554/eLife.82979.
10
BleTIES: annotation of natural genome editing in ciliates using long read sequencing.BleTIES：使用长读测序对纤毛虫中的自然基因组编辑进行注释。
Bioinformatics. 2021 Nov 5;37(21):3929-3931. doi: 10.1093/bioinformatics/btab613.
本文引用的文献
1
A micronucleus-specific sequence exists in the 5'-upstream region of calmodulin gene in Tetrahymena thermophila.嗜热四膜虫钙调蛋白基因5'-上游区域存在一个微核特异性序列。
Nucleic Acids Res. 1993 May 25;21(10):2409-14. doi: 10.1093/nar/21.10.2409.
2
Circular forms of developmentally excised DNA in Euplotes crassus have a heteroduplex junction.粗多甲藻中发育切除的环状DNA形式具有异源双链体连接。
Genes Dev. 1993 Jan;7(1):84-94. doi: 10.1101/gad.7.1.84.
3
The mariner transposable element is widespread in insects.水手转座元件在昆虫中广泛存在。
Nature. 1993 Mar 18;362(6417):241-5. doi: 10.1038/362241a0.
4
Elimination of Tec elements involves a novel excision process.Tec 元件的消除涉及一种新的切除过程。
Genes Dev. 1993 Jan;7(1):95-105. doi: 10.1101/gad.7.1.95.
5
A proposed superfamily of transposase genes: transposon-like elements in ciliated protozoa and a common "D35E" motif.转座酶基因的一个拟议超家族：纤毛原生动物中的类转座子元件及一个常见的“D35E”基序
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):942-6. doi: 10.1073/pnas.91.3.942.
6
7
A distant 10-bp sequence specifies the boundaries of a programmed DNA deletion in Tetrahymena.一段距离较远的10个碱基对的序列确定了四膜虫中一个程序化DNA缺失的边界。
Genes Dev. 1993 Dec;7(12A):2357-65. doi: 10.1101/gad.7.12a.2357.
8
Structures of the Euplotes crassus Tec1 and Tec2 elements: identification of putative transposase coding regions.粗壮真核游仆虫Tec1和Tec2元件的结构：推定转座酶编码区域的鉴定
Gene. 1993 Oct 29;133(1):71-8. doi: 10.1016/0378-1119(93)90226-s.
9
Developmental precise excision of Oxytricha trifallax telomere-bearing elements and formation of circles closed by a copy of the flanking target duplication.嗜热四膜虫含端粒元件的发育精确切除及由侧翼靶标重复序列的一个拷贝封闭形成环状结构
EMBO J. 1993 Dec;12(12):4593-601. doi: 10.1002/j.1460-2075.1993.tb06148.x.
10
Developmentally excised sequences in micronuclear DNA of Paramecium.草履虫微核DNA中发育过程中切除的序列。
Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2255-9. doi: 10.1073/pnas.91.6.2255.
Zotero 插件