Dittmann K, Löffler H, Bamberg M, Rodemann H P
Department of Radiotherapy, Eberhard-Karls-University, Tübingen, Germany.
Radiother Oncol. 1995 Feb;34(2):137-43. doi: 10.1016/0167-8140(94)01494-n.
The radiosensitivity and differentiation pattern of cultured normal human fibroblasts was analysed as a function of treatment of the cells with the Bowman-Birk proteinase inhibitor (BBI). Upon irradiation with doses from 0 to 8 Gy normal human fibroblasts are induced to a premature terminal differentiation within 14-21 days of postirradiation incubation. Treatment of the cells with 10 microM BBI for 2 h prior to the irradiation procedure resulted in a significant shift of the radiation survival curve, increased SF2 values 0.63 vs. 0.84 and the cell type composition of the test fibroblast cultures. Upon pretreatment with BBI the radiation-induced premature terminal differentiation of progenitor fibroblasts to postmitotic fibrocytes could significantly be inhibited. Based on this data, it can be postulated that BBI may serve as a radioprotector of normal fibroblasts which are involved in radiation-induced tissue injuries like radiation fibrosis.
作为用鲍曼-伯克蛋白酶抑制剂（BBI）处理细胞的函数，分析了培养的正常人成纤维细胞的放射敏感性和分化模式。用0至8 Gy剂量照射后，正常人成纤维细胞在照射后培养14 - 21天内被诱导进入过早的终末分化。在照射程序前用10μM BBI处理细胞2小时，导致放射存活曲线显著偏移，SF2值增加（0.63对0.84）以及测试成纤维细胞培养物的细胞类型组成发生变化。用BBI预处理后，祖细胞成纤维细胞向有丝分裂后纤维细胞的辐射诱导过早终末分化可被显著抑制。基于这些数据，可以推测BBI可能作为正常成纤维细胞的辐射防护剂，正常成纤维细胞参与如放射性纤维化等辐射诱导的组织损伤。
