使用DNA探针鉴定日本东部和中部分离出的鸟分枝杆菌复合体。
Identification of Mycobacterium avium complex isolated in eastern and central Japan by using DNA probes.
作者信息
Watanabe A, Kikuchi H, Shoji S, Nukiwa T, Motomiya M, Yoshida T, Takizawa S, Oizumi K
机构信息
Department of Respiratory Medicine, Tohoku University, Sendai, Japan.
出版信息
Tohoku J Exp Med. 1995 Feb;175(2):139-42. doi: 10.1620/tjem.175.139.
An attempt was made to identify Mycobacterium avium and Mycobacterium intracellulare in the M. avium intracellulare complex (MAC) isolated in the Tohoku (38 strains) and Tokai (30 strains) districts of Japan by using DNA probes which are specific for M. avium, M. intracellulare and M. tuberculosis complex, respectively. The incidence of M. avium infection (82%) by far exceeded that of M. intracellulare infection in the Tohoku district of eastern Japan. In the Tokai district of central Japan, the incidence of M. avium infection (57%) were slightly larger than that of M. intracellulare infection. Five of 68 strains showed a positive reaction with two different DNA probes. Thus a possibility of mixed infection could not be ruled out, because reference strains showed a positive reaction with only one species-specific DNA probe.
利用分别针对鸟分枝杆菌、胞内分枝杆菌和结核分枝杆菌复合群的DNA探针，对从日本东北地区（38株）和东海地区（30株）分离出的鸟分枝杆菌胞内分枝杆菌复合群（MAC）中的鸟分枝杆菌和胞内分枝杆菌进行鉴定。在日本东部的东北地区，鸟分枝杆菌感染的发生率（82%）远远超过胞内分枝杆菌感染的发生率。在日本中部的东海地区，鸟分枝杆菌感染的发生率（57%）略高于胞内分枝杆菌感染的发生率。68株菌株中有5株对两种不同的DNA探针呈阳性反应。因此，不能排除混合感染的可能性，因为参考菌株仅对一种种特异性DNA探针呈阳性反应。
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