Jensen-Waern M, Johannisson A, Ederoth M, Trowald-Wigh G
Department of Medicine and Surgery, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Zentralbl Veterinarmed B. 1994 Dec;41(10):625-38. doi: 10.1111/j.1439-0450.1994.tb00273.x.
The adhesive and phagocytic properties of porcine neutrophils were studied. Blood samples were collected from healthy crossbreed pigs, and granulocytes were obtained by density centrifugation. Adhesion to nylon wool was studied and expression of adhesion molecules was determined by flow cytometry. Three monoclonal antibodies showed reactivity with porcine cells and were thus subsequently used. Freezing and storage at -70 degrees C did not cause major alterations in the expression of adhesion molecules. The kinetics of phagocytosis of serum- and IgG-opsonized yeast cells were studied with fluorescence microscopy and flow cytometry. The influence of various concentrations of normal pooled and heat-inactivated pooled porcine serum on the phagocytic process was elucidated. The phagocytic process was completed more rapidly using C3b- than IgG-opsonized yeast cells. The neutrophils both attached to and ingested the serum-opsonized yeast cells to a large extent. When IgG or heat-inactivated serum were used, only minor attachment was observed. These methods for studies of neutrophil functions can be used both for the diagnosis of immunological disorders and for physiological studies of the neutrophil functions in the pig.
对猪中性粒细胞的黏附及吞噬特性进行了研究。从健康杂交猪采集血样，通过密度离心法获得粒细胞。研究了对尼龙毛的黏附情况，并通过流式细胞术测定黏附分子的表达。三种单克隆抗体与猪细胞有反应性，因此随后被使用。在-70℃冷冻保存并未导致黏附分子表达发生重大改变。用荧光显微镜和流式细胞术研究了血清和IgG调理的酵母细胞的吞噬动力学。阐明了不同浓度的正常混合及热灭活混合猪血清对吞噬过程的影响。使用C3b调理的酵母细胞比IgG调理的酵母细胞吞噬过程完成得更快。中性粒细胞在很大程度上既附着又摄取了血清调理的酵母细胞。当使用IgG或热灭活血清时，仅观察到轻微的附着。这些研究中性粒细胞功能的方法可用于免疫紊乱的诊断以及猪中性粒细胞功能的生理学研究。
