用于检测葡萄球菌肠毒素B的采用鼠单克隆抗体的双夹心酶联免疫吸附测定（ELISA）
Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B.
作者信息
Goyache J, Orden J A, Blanco J L, Doménech A, Suárez G, Gómez-Lucía E
机构信息
Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
出版信息
Zentralbl Veterinarmed B. 1994 Dec;41(10):639-44. doi: 10.1111/j.1439-0450.1994.tb00274.x.
Three monoclonal antibodies (MAbs) which react specifically with staphylococcal enterotoxin B (SEB) were studied for their suitability for use in ELISA. One of them (MAb B14) worked well as a coating antibody; MAb B12 was shown to be a good probing antibody (conjugated with peroxidase) when ELISA plates were coated with MAb B14. This effective pair of MAbs (B14-B12PO) is able to detect 0.625 ng SEB ml, and to distinguish between SEB and other proteins present in food extracts.
研究了三种与葡萄球菌肠毒素B（SEB）特异性反应的单克隆抗体（MAb）用于酶联免疫吸附测定（ELISA）的适用性。其中一种（单克隆抗体B14）作为包被抗体效果良好；当ELISA板用单克隆抗体B14包被时，单克隆抗体B12被证明是一种很好的检测抗体（与过氧化物酶偶联）。这一有效的单克隆抗体对（B14-B12PO）能够检测出0.625纳克/毫升的SEB，并能区分SEB与食品提取物中存在的其他蛋白质。
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