[竹红菌素B-乙醇胺（HB-E）对大鼠线粒体的敏化光损伤]

[Hypocrellin B-ethanolamine (HB-E) sensitized photodamage on rat mitochondria].

作者信息

Yue J C, Liu M, Wan P, Pang S Z

机构信息

Institute of Biophysics, Academia Sinica, Beijing, China.

出版信息

Shi Yan Sheng Wu Xue Bao. 1995 Mar;28(1):23-30.

PMID:7597866

Abstract

The Hypocrellin B-Ethanolamine (HB-E) sensitized photodamage on lipid peroxidation, sulfhydryl oxidation and ATPase inactivation of rat liver mitochondria was studied under illumination of 640 nm, The HB-E sensitized photodamage of lipid peroxidation is less than sulfhydryl photooxidation, when HB-E concentration is 50 mumol/L. To elucidate the mechanism of photodamage of HB-E, antioxidant: BHT, Vit E, oxygen radical quencher: L-His, beta-Cart, were investigated. The results showed that HB photodamage of mitochondria is not only by oxygen radical, but also by HB-E radical as well. From compared tests among HB, HA, Hp, MB and HB-E, we found that HB-E had higher ability to photodamage lipid peroxidation, sulfhydryl oxidation and tryptophan than other photosensitizers. The experiments showed that the superoxide generated may be by HB-E radical.

摘要

研究了竹红菌素B -乙醇胺（HB - E）在640nm光照下对大鼠肝线粒体脂质过氧化、巯基氧化和ATP酶失活的敏化光损伤作用。当HB - E浓度为50μmol/L时，其敏化脂质过氧化的光损伤作用小于巯基光氧化作用。为阐明HB - E光损伤的机制，研究了抗氧化剂：丁基羟基甲苯（BHT）、维生素E（Vit E），氧自由基猝灭剂：L -组氨酸（L - His）、β -胡萝卜素（β - Cart）。结果表明，HB对线粒体的光损伤不仅通过氧自由基，还通过HB - E自由基。通过对HB、竹红菌素A（HA）、竹红菌丙素（Hp）、亚甲基蓝（MB）和HB - E的对比试验，发现HB - E比其他光敏剂对脂质过氧化、巯基氧化和色氨酸的光损伤能力更强。实验表明，超氧阴离子可能由HB - E自由基产生。