利用肌红蛋白信号通过核磁共振氢谱法观察组织氧合作用。

1H NMR approach to observe tissue oxygenation with the signals of myoglobin.

作者信息

Jue T, Kreutzer U, Chung Y

机构信息

Biological Chemistry Department, University of California Davis 95616-8635, USA.

出版信息

Adv Exp Med Biol. 1994;361:111-8. doi: 10.1007/978-1-4615-1875-4_15.

DOI:10.1007/978-1-4615-1875-4_15

PMID:7597933

Abstract

The myoglobin technique measures oxygen tension in myocytes. It relies on a quantitative measurement of the Val E11 and His F8 signals and an accurate value for the [O2]50 for Mb. Even though the Mb oxygen affinity in the cell is in question, the NMR results still reflect the degree of Mb oxygen saturation. Although magnetic resonance has established a variety of strategies to measure tissue oxygenation, the Mb approach is the most direct and will lead to a better understanding of oxygen's role in regulating cellular activity.

摘要

肌红蛋白技术用于测量心肌细胞中的氧张力。它依赖于对缬氨酸E11和组氨酸F8信号的定量测量以及肌红蛋白（Mb）的[O2]50准确值。尽管细胞中肌红蛋白的氧亲和力存在疑问，但核磁共振结果仍能反映肌红蛋白的氧饱和度。虽然磁共振已建立多种测量组织氧合的策略，但肌红蛋白方法是最直接的，将有助于更好地理解氧在调节细胞活动中的作用。