组织肌红蛋白的1H核磁共振观察：体内细胞氧合的一个指标。
1H NMR observation of tissue myoglobin: an indicator of cellular oxygenation in vivo.
作者信息
Jue T, Anderson S
机构信息
Department of Biological Chemistry, University of California, Davis 95616.
出版信息
Magn Reson Med. 1990 Mar;13(3):524-8. doi: 10.1002/mrm.1910130322.
Cellular oxygen status is a critical parameter. To measure the oxygen tension in vivo, however, demands extant techniques to surmount two experimental difficulties: invasive experimental protocols and imprecise tissue localization. NMR techniques have the potential to overcome these obstacles. Indeed we show in this study that cellular myoglobin is NMR visible and its proximal histidyl NH signal in the deoxy state can be used to monitor changes in cellular oxygenation. Coupled with recent advances in NMR signal localization, this 1H NMR strategy promises to lead directly to cellular oxygen measurement in specific tissue regions in vivo.
细胞氧状态是一个关键参数。然而，要测量体内的氧张力，现有的技术需要克服两个实验难题：侵入性实验方案和不精确的组织定位。核磁共振（NMR）技术有潜力克服这些障碍。事实上，我们在本研究中表明，细胞肌红蛋白在核磁共振中是可见的，其脱氧状态下近端组氨酸NH信号可用于监测细胞氧合的变化。结合核磁共振信号定位的最新进展，这种1H NMR策略有望直接实现体内特定组织区域的细胞氧测量。
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