Krizanova O, Varadi M, Schwartz A, Varadi G
Institute of Molecular Pharmacology and Biophysics, University of Cincinnati, OH 45267-0828, USA.
Biochem Biophys Res Commun. 1995 Jun 26;211(3):921-7. doi: 10.1006/bbrc.1995.1900.
Coexpression of alpha 1 and beta subunits for all tissue-specific isotypes of calcium channels results in an increase in Ca2+ channel current density and drug receptor function. The quantification of the photoaffinity labeled skeletal muscle alpha 1 subunit polypeptide by two alternative immunoadsorption techniques showed that 3 times higher amount of calcium channel alpha 1 protein appears in the cell membrane when alpha 1 and beta subunits were coexpressed compared to alpha 1 alone. Using Western blotting and immunodetection techniques, we identified two polypeptide bands of alpha 1 with 210 kDa and 170 kD molecular mass, respectively. The combined intensity of these bands was 7-9 times higher when alpha 1 and beta were coexpressed compared to alpha 1 alone. The data provide evidence that coexpression of alpha 1 and beta results in an increased amount of alpha 1 protein in the cell membrane.
钙通道所有组织特异性同种型的α1和β亚基共表达会导致Ca2+通道电流密度和药物受体功能增加。通过两种替代免疫吸附技术对光亲和标记的骨骼肌α1亚基多肽进行定量分析表明，与单独表达α1相比，当α1和β亚基共表达时，细胞膜中出现的钙通道α1蛋白量高出3倍。使用蛋白质印迹法和免疫检测技术，我们分别鉴定出两条分子量为210 kDa和170 kD的α1多肽条带。与单独表达α1相比，当α1和β共表达时，这些条带的综合强度高出7 - 9倍。这些数据提供了证据，表明α1和β的共表达会导致细胞膜中α1蛋白量增加。
